关键信息
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基因名
HAL
- 应用
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别名
IS; hutH 1; Hal; HAL protein; Histidase; Histidine ammonia lyase; Histidine ammonia-lyase; HSTD; HutH; HUTH_HUMAN
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P42357
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表达区间
1-657aa
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氨基酸序列
MPRYTVHVRGEWLAVPCQDAQLTVGWLGREAVRRYIKNKPDNGGFTSVDDAHFLVRRCKGLGLLDNEDRLEVALENNEFVEVVIEGDAMSPDFIPSQPEGVYLYSKYREPEKYIELDGDRLTTEDLVNLGKGRYKIKLTPTAEKRVQKSREVIDSIIKEKTVVYGITTGFGKFARTVIPINKLQELQVNLVRSHSSGVGKPLSPERCRMLLALRINVLAKGYSGISLETLKQVIEMFNASCLPYVPEKGTVGASGDLAPLSHLALGLVGEGKMWSPKSGWADAKYVLEAHGLKPVILKPKEGLALINGTQMITSLGCEAVERASAIARQADIVAALTLEVLKGTTKAFDTDIHALRPHRGQIEVAFRFRSLLDSDHHPSEIAESHRFCDRVQDAYTLRCCPQVHGVVNDTIAFVKNIITTELNSATDNPMVFANRGETISGGNFHGEYPAKALDYLAIGIHELAAISERRIERLCNPSLSELPAFLVAEGGLNSGFMIAHCTAAALVSENKALCHPSSVDSLSTSAATEDHVSMGGWAARKALRVIEHVEQVLAIELLAACQGIEFLRPLKTTTPLEKVYDLVRSVVRPWIKDRFMAPDIEAAHRLLLEQKVWEVAAPYIEKYRMEHIPESRPLSPTAFSLQFLHKKSTKIPESEDL
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分子量
99.1 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
HAL (Histamine N-Methyltransferase) is an enzyme involved in the metabolism of histamine, a biogenic amine that plays crucial roles in various physiological processes, including immune response, gastric acid secretion, and neurotransmission. The research on HAL recombined proteins has gained prominence due to their potential therapeutic applications in managing allergic reactions, neurological disorders, and gastric diseases. By understanding the structure and function of HAL, researchers aim to develop novel interventions that can modulate histamine levels in the body. Recombinant HAL proteins can be produced using advanced biotechnological methods, allowing for the study of enzyme kinetics, substrate specificity, and the development of inhibitors or activators. Furthermore, as histamine-related disorders become increasingly prevalent in modern society, the demand for efficient treatments underscores the importance of HAL in biomedical research. Investigating HAL’s role in disease mechanisms also opens new avenues for drug discovery and personalized medicine, making it a critical target for therapeutic exploration. Thus, the study of HAL recombined proteins not only enhances our understanding of histamine metabolism but also holds promise for clinical advancements in the treatment of several histamine-related health issues.
