关键信息
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基因名
GATC
- 应用
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别名
GATC；15E1.2;Glutamyl-tRNA(Gln) amidotransferase subunit C. mitochondrial
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O43716
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表达区间
1-136aa
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氨基酸序列
MGSSHHHHHH SSGLVPRGSH MGSMWSRLVW LGLRAPLGGR QGFTSKADPQ GSGRITAAVI EHLERLALVD FGSREAVARL EKAIAFADRL RAVDTDGVEP MESVLEDRCL YLRSDNVVEG NCADELLQNS HRVVEEYFVA PPGNISLPKL DEQEPFPHS
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分子量
18 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
GATC recombinant proteins have garnered significant interest in biochemical and molecular biology research due to their role in various cellular processes and potential applications in biotechnology. GATC refers to a specific DNA sequence that contains the adenine nucleotide (A) methylated at the N6 position, which is characterized by the presence of guanine (G), adenine (A), thymine (T), and cytosine (C) in a particular order. This sequence is recognized by the Dam methyltransferase, an enzyme that plays a crucial role in DNA replication, repair, and gene expression regulation. The study of GATC recombinant proteins focuses on understanding the dynamics of DNA-protein interactions, the mechanisms of methylation and demethylation, and their implications in epigenetic modifications. Researchers are particularly interested in the potential therapeutic applications of manipulating GATC methylation patterns, as they may influence gene expression and cellular behavior in various diseases, including cancer. By expressing GATC-related proteins in recombinant systems, scientists can investigate their functional roles, enhance our understanding of genetic regulation, and explore novel approaches for gene editing and targeted therapies. This research is vital for unraveling the complexities of epigenetic regulation and developing innovative biotechnological applications, thereby contributing to advancements in medicine, agriculture, and synthetic biology.
