| 纯度 | > 85 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | BTF3 |
| Uniprot No | P20290 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-206aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMRRTGAP AQADSRGRGR ARGGCPGGEA TLSQPPPRGG TRGQEPQMKE TIMNQEKLAK LQAQVRIGGK GTARRKKKVV HRTATADDKK LQFSLKKLGV NNISGIEEVN MFTNQGTVIH FNNPKVQASL AANTFTITGH AETKQLTEML PSILNQLGAD SLTSLRRLAE ALPKQSVDGK APLATGEDDD DEVPDLVENF DEASKNEAN |
| 预测分子量 | 25 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与BTF3重组蛋白相关的参考文献摘要信息：
1. **《BTF3基因的克隆及重组蛋白在大肠杆菌中的表达》**
作者：王XX等(2020)
摘要：研究通过PCR技术从人类cDNA文库中克隆BTF3基因，成功构建pET28a-BTF3重组质粒，利用大肠杆菌BL21(DE3)进行诱导表达，通过镍柱纯化获得高纯度重组蛋白，为后续功能研究奠定基础。
2. **《重组BTF3蛋白在肝癌细胞凋亡调控中的作用》**
作者：李X等(2018)
摘要：通过原核表达系统制备重组BTF3蛋白，发现其能显著抑制肝癌细胞HepG2的增殖并诱导凋亡，Western blot显示其通过下调Bcl-2和激活caspase-3通路发挥作用。
3. **《BTF3重组蛋白与DNA结合特性的生物信息学及实验验证》**
作者：Smith J. et al. (2016)
摘要：利用分子对接预测BTF3重组蛋白的DNA结合域，并通过凝胶迁移实验(EMSA)证实其与靶基因启动子区特异性结合，揭示其在转录调控中的分子机制。
注：以上为虚构参考文献，实际研究中建议通过PubMed或Web of Science以"BTF3 recombinant protein"等关键词检索真实文献。
**Background of BTF3 Recombinant Protein**
The **BTF3 (Basic Transcription Factor 3)** protein, also known as **NACA (Nascent Polypeptide-Associated Complex Alpha subunit)**, is a multifunctional regulator involved in transcription, translation, and protein quality control. Initially identified as a component of the nascent polypeptide-associated complex (NAC), BTF3/NACA binds to emerging polypeptide chains during translation, preventing inappropriate interactions with other cellular factors. It also plays a role in transcriptional regulation by modulating RNA polymerase II activity, thereby influencing gene expression.
Recombinant BTF3 protein is produced using genetic engineering techniques, typically expressed in *E. coli* or mammalian cell systems*. This allows for high-purity, scalable production of the protein for research and therapeutic applications. The recombinant form retains functional domains critical for its interaction with ribosomes, transcription machinery, and chaperone systems.
Studies highlight BTF3's involvement in cellular stress responses, cancer progression, and neurodegenerative diseases. For instance, elevated BTF3 levels correlate with tumor growth and metastasis in certain cancers, potentially serving as a biomarker. Its role in mitigating protein aggregation also links it to neurodegenerative conditions like Alzheimer’s disease.
In research, recombinant BTF3 is utilized to explore mechanisms of gene expression, protein synthesis, and stress adaptation. It aids in structural studies, drug screening, and understanding disease pathways. Its dual functionality in transcription and translation makes it a unique tool for dissecting cross-talk between these processes.
Overall, BTF3 recombinant protein is a valuable resource for advancing molecular biology and translational medicine, offering insights into fundamental cellular processes and disease mechanisms.
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