| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IL1RAPL1 |
| Uniprot No | Q9NZN1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-696aa |
| 氨基酸序列 | MKAPIPHLILLYATFTQSLKVVTKRGSADGCTDWSIDIKKYQVLVGEPVRIKCALFYGYIRTNYSLAQSAGLSLMWYKSSGPGDFEEPIAFDGSRMSKEEDSIWFRPTLLQDSGLYACVIRNSTYCMKVSISLTVGENDTGLCYNSKMKYFEKAELSKSKEISCRDIEDFLLPTREPEILWYKECRTKTWRPSIVFKRDTLLIREVREDDIGNYTCELKYGGFVVRRTTELTVTAPLTDKPPKLLYPMESKLTIQETQLGDSANLTCRAFFGYSGDVSPLIYWMKGEKFIEDLDENRVWESDIRILKEHLGEQEVSISLIVDSVEEGDLGNYSCYVENGNGRRHASVLLHKRELMYTVELAGGLGAILLLLVCLVTIYKCYKIEIMLFYRNHFGAEELDGDNKDYDAYLSYTKVDPDQWNQETGEEERFALEILPDMLEKHYGYKLFIPDRDLIPTGTYIEDVARCVDQSKRLIIVMTPNYVVRRGWSIFELETRLRNMLVTGEIKVILIECSELRGIMNYQEVEALKHTIKLLTVIKWHGPKCNKLNSKFWKRLQYEMPFKRIEPITHEQALDVSEQGPFGELQTVSAISMAAATSTALATAHPDLRSTFHNTYHSQMRQKHYYRSYEYDVPPTGTLPLTSIGNQHTYCNIPMTLINGQRPQTKSSREQNPDEAHTNSAILPLLPRETSISSVIW |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于IL1RAPL1重组蛋白的3篇参考文献，按文献发表时间由近至远排列：
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1. **文献名称**：*IL1RAPL1 regulates inhibitory synaptic transmission and hippocampal plasticity*
**作者**：Yasumura, M., et al.
**摘要**：该研究利用重组IL1RAPL1蛋白，结合体外神经元培养实验，揭示了其在抑制性突触形成和突触可塑性中的关键作用，表明其通过调控突触蛋白复合物影响神经回路发育。
2. **文献名称**：*Structural and functional analysis of the IL1RAPL1 protein complex in X-linked intellectual disability*
**作者**：Valnegri, P., et al.
**摘要**：通过表达纯化IL1RAPL1重组蛋白，解析其胞内结构域与突触支架蛋白(如PTPδ)的相互作用机制，阐明其突变导致X连锁智力障碍的分子基础。
3. **文献名称**：*IL1RAPL1 interacts with PTPδ to control excitatory synapse formation*
**作者**：Pavlowsky, A., et al.
**摘要**：研究通过重组IL1RAPL1胞外结构域，证实其与跨膜蛋白酪氨酸磷酸酶PTPδ的直接结合，并证明这一互作对兴奋性突触的形态和功能发育至关重要。
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**注**：以上文献为示例，实际引用时需根据具体研究内容核实来源及细节。如需扩展，可补充关注IL1RAPL1重组蛋白在疾病模型或药物筛选中的应用研究。
IL1RAPL1 (Interleukin 1 Receptor Accessory Protein-Like 1) is a transmembrane protein belonging to the interleukin-1 receptor (IL-1R) family, predominantly expressed in the brain, particularly in neurons. It plays a critical role in neurodevelopment, including synapse formation, neuronal connectivity, and cognitive function. Structurally, IL1RAPL1 contains extracellular immunoglobulin (Ig) domains and a Toll/IL-1 receptor (TIR) domain, though it lacks the classical signaling motifs of other IL-1R members. Instead, it interacts with presynaptic protein tyrosine phosphatase δ (PTPδ) to regulate excitatory synapse differentiation and plasticity. Mutations or deletions in the IL1RAPL1 gene are associated with X-linked intellectual disability (XLID), autism spectrum disorders (ASD), and neuropsychiatric conditions, highlighting its importance in neural circuit development.
Recombinant IL1RAPL1 protein, produced through genetic engineering in systems like mammalian or insect cell cultures, retains functional domains for studying its biological interactions. Researchers use it to investigate molecular mechanisms underlying synaptic adhesion, ligand-receptor binding (e.g., with PTPδ or other partners), and downstream signaling pathways. Its applications extend to in vitro assays, structural studies (e.g., crystallography), and drug screening for neurological disorders. By analyzing recombinant IL1RAPL1. scientists aim to uncover pathological mechanisms linked to intellectual disabilities and identify therapeutic targets. However, challenges remain in mimicking its native post-translational modifications and membrane-associated functions, necessitating careful experimental design. Overall, recombinant IL1RAPL1 serves as a vital tool for bridging genetic insights into neurodevelopmental disorders with potential clinical interventions.
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