| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Arachidonate 12-lipoxygenase, 12R-type, 12R-LOX, 12R-lipoxygenase, 11311-, Epidermis-type lipoxygenase 12, ALOX12B |
| Entrez GeneID | 242 |
| WB Predicted band size | 80.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This ALOX12B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 622-652 amino acids from the C-terminal region of human ALOX12B. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于ALOX12B抗体的3篇示例文献的简要信息(注：以下内容为示例性概括，实际文献需通过学术数据库查询确认)：
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1. **文献名称**: *"ALOX12B mutations in congenital ichthyosis: Functional characterization of a missense variant and development of a monoclonal antibody for protein analysis"*
**作者**: Smith J, et al.
**摘要**: 研究报道了ALOX12B基因突变与先天性鱼鳞病的关联，开发了一种特异性单克隆抗体用于检测ALOX12B蛋白表达，并通过免疫印迹和免疫组化验证其在患者皮肤组织中的表达缺失。
2. **文献名称**: *"Lipoxygenase ALOX12B structure and activity revealed by antibody-mediated epitope mapping"*
**作者**: Chen L, et al.
**摘要**: 通过抗体制备和表位定位技术解析ALOX12B的活性结构域，发现其催化功能依赖特定保守区域，抗体阻断实验表明ALOX12B在表皮脂质代谢中的关键作用。
3. **文献名称**: *"Autoantibodies against ALOX12B in autoimmune skin disorders: Diagnostic implications"*
**作者**: Gupta R, et al.
**摘要**: 研究检测到部分自身免疫性皮肤病患者血清中存在抗ALOX12B自身抗体，提示该抗体可能作为疾病生物标志物，并探讨了其与表皮屏障损伤的相关性。
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**建议**：以上为模拟示例，实际文献请通过PubMed、Web of Science或Google Scholar检索关键词(如“ALOX12B antibody”“ALOX12B in skin disease”)获取最新研究。
The ALOX12B antibody is designed to target the ALOX12B enzyme, also known as 12R-lipoxygenase (12R-LOX), which is encoded by the *ALOX12B* gene. This enzyme belongs to the lipoxygenase family and catalyzes the oxidation of arachidonic acid to specific hydroperoxy metabolites, playing a role in epidermal differentiation and skin barrier formation. ALOX12B is primarily expressed in epithelial tissues, particularly the skin, where it contributes to the synthesis of lipids critical for maintaining the stratum corneum structure.
Mutations in *ALOX12B* are linked to autosomal recessive congenital ichthyosis (ARCI), a group of severe skin disorders characterized by impaired desquamation and barrier dysfunction. These mutations disrupt enzyme activity, leading to defective lipid metabolism and abnormal epidermal maturation. Research on ALOX12B antibodies focuses on elucidating the enzyme's role in skin biology, disease mechanisms, and potential therapeutic targets. These antibodies are used in techniques like immunohistochemistry, Western blotting, and immunofluorescence to assess protein expression, localization, and dysfunction in cellular or tissue models. Additionally, they aid in studying genotype-phenotype correlations in ichthyosis patients and evaluating experimental therapies aimed at restoring lipid metabolism. Beyond dermatology, ALOX12B has been explored in inflammatory and cancer pathways due to its involvement in bioactive lipid signaling. The antibody thus serves as a vital tool in both basic research and clinical diagnostics.
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