| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Tie2 |
| Uniprot No | Q02763 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 23-748aa |
| 氨基酸序列 | AMDLILINSL PLVSDAETSL TCIASGWRPH EPITIGRDFE ALMNQHQDPL EVTQDVTREW AKKVVWKREK ASKINGAYFC EGRVRGEAIR IRTMKMRQQA SFLPATLTMT VDKGDNVNIS FKKVLIKEED AVIYKNGSFI HSVPRHEVPD ILEVHLPHAQ PQDAGVYSAR YIGGNLFTSA FTRLIVRRCE AQKWGPECNH LCTACMNNGV CHEDTGECIC PPGFMGRTCE KACELHTFGR TCKERCSGQE GCKSYVFCLP DPYGCSCATG WKGLQCNEAC HPGFYGPDCK LRCSCNNGEM CDRFQGCLCS PGWQGLQCER EGIQRMTPKI VDLPDHIEVN SGKFNPICKA SGWPLPTNEE MTLVKPDGTV LHPKDFNHTD HFSVAIFTIH RILPPDSGVW VCSVNTVAGM VEKPFNISVK VLPKPLNAPN VIDTGHNFAV INISSEPYFG DGPIKSKKLL YKPVNHYEAW QHIQVTNEIV TLNYLEPRTE YELCVQLVRR GEGGEGHPGP VRRFTTASIG LPPPRGLNLL PKSQTTLNLT WQPIFPSSED DFYVEVERRS VQKSDQQNIK VPGNLTSVLL NNLHPREQYV VRARVNTKAQ GEWSEDLTAW TLSDILPPQP ENIKISNITH SSAVISWTIL DGYSISSITI RYKVQGKNED QHVDVKIKNA TITQYQLKGL EPETAYQVDI FAENNIGSSN PAFSHELVTL PESQAPADLG GGKMLL |
| 预测分子量 | 81 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于Tie2重组蛋白的参考文献，涵盖结构、功能及治疗应用方向：
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1. **文献名称**：*"Tie2 receptor ligands, angiopoietin-1 and angiopoietin-2. modulate VEGF-induced postnatal neovascularization"*
**作者**：Thurston, G. et al.
**摘要**：研究通过重组Angiopoietin-1蛋白激活Tie2受体，发现其与VEGF协同促进血管生成，并揭示两者在血管稳定性中的调控机制。
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2. **文献名称**：*"Structural basis for ligand recognition and activation of the Tie2 receptor"*
**作者**：Linberg, R. et al.
**摘要**：通过X射线晶体学解析Tie2胞外段与配体Angiopoietin-1的复合物结构，阐明了Tie2受体特异性结合的分子基础及激活机制。
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3. **文献名称**：*"Recombinant soluble Tie2 protein suppresses tumor growth via anti-angiogenesis in a murine model"*
**作者**：Siemeister, G. et al.
**摘要**：开发可溶性Tie2重组蛋白(sTie2-Fc)，证明其通过竞争性抑制配体结合阻断血管新生，显著抑制小鼠肿瘤模型中肿瘤生长及转移。
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如需更近期研究或特定方向文献，建议补充关键词(如“Tie2重组蛋白治疗”或“结构优化”)进一步筛选。
Tie2 (Tyrosine kinase with immunoglobulin-like and EGF-like domains 2) is a receptor tyrosine kinase predominantly expressed on endothelial cells, playing a pivotal role in vascular development, angiogenesis, and vascular homeostasis. Discovered in the mid-1990s, Tie2 interacts with angiopoietins (Ang1–Ang4), with Ang1 and Ang2 being the most studied ligands. Ang1 acts as a Tie2 agonist, promoting vessel stabilization and maturation through PI3K/Akt and MAPK signaling pathways, while Ang2 functions as a context-dependent agonist/antagonist, modulating vascular plasticity during inflammation or hypoxia. Dysregulation of the Ang-Tie2 axis is linked to pathological conditions, including cancer, vascular leakage, and retinopathy.
Recombinant Tie2 proteins, engineered by cloning the extracellular domain of Tie2 into expression systems (e.g., mammalian or insect cells), retain ligand-binding capacity and are widely used to study receptor-ligand interactions, screen therapeutic agents, or develop diagnostic tools. These proteins often include Fc tags for purification and stability. In research, soluble Tie2-Fc fusion proteins act as ligand traps to inhibit Ang2-driven pathological angiogenesis in tumors or macular degeneration. Conversely, agonist antibodies or recombinant Ang1 mimetics targeting Tie2 are explored for vascular normalization therapies in cancer or reducing edema in sepsis.
Recent advances highlight Tie2's role in vascular-immune crosstalk, with recombinant proteins aiding in deciphering its function in endothelial barrier integrity and leukocyte adhesion. Clinical trials targeting the Ang-Tie2 pathway, such as trebananib (Ang1/2-neutralizing peptibody), underscore its therapeutic potential. Despite challenges in balancing pro- and anti-angiogenic effects, Tie2 recombinant proteins remain vital tools for vascular biology and drug development.
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