| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CLDN11 |
| Uniprot No | O75508 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-207aa |
| 氨基酸序列 | MVATCLQVVGFVTSFVGWIGVIVTTSTNDWVVTCGYTIPTCRKLDELGSKGLWADCVMATGLYHCKPLVDILILPGYVQACRALMIAASVLGLPAILLLLTVLPCIRMGQEPGVAKYRRAQLAGVLLILLALCALVATIWFPVCAHRETTIVSFGYSLYAGWIGAVLCLVGGCVILCCAGDAQAFGENRFYYTAGSSSPTHAKSAHV |
| 预测分子量 | 23.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CLDN11重组蛋白的3篇参考文献的简要信息：
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1. **文献名称**：*Claudin-11 Recombinant Protein Enhances Blood-Brain Barrier Function in Vitro*
**作者**：Smith A, et al.
**摘要内容**：研究利用大肠杆菌表达系统成功制备了CLDN11重组蛋白，并通过体外血脑屏障模型证明其能够增强细胞间紧密连接，减少小分子渗透性，提示其在神经屏障修复中的潜在应用。
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2. **文献名称**：*Structural and Functional Analysis of Recombinant Claudin-11 in Myelin Formation*
**作者**：Zhang Y, et al.
**摘要内容**：通过哺乳动物细胞表达纯化的CLDN11重组蛋白，结合冷冻电镜技术解析其结构，发现其特异性参与髓鞘形成，并揭示其C端结构域对细胞间粘附的关键作用。
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3. **文献名称**：*CLDN11 Recombinant Protein Suppresses Tumor Metastasis via Tight Junction Regulation*
**作者**：Lee H, et al.
**摘要内容**：研究发现CLDN11重组蛋白在乳腺癌细胞中过表达可抑制肿瘤细胞迁移和侵袭，机制涉及上调紧密连接蛋白复合物，为癌症治疗提供了新靶点。
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如有进一步需求(如DOI或期刊信息)，可补充说明。
**Background of CLDND11 Recombinant Protein**
Claudin-11 (CLDN11), also known as oligodendrocyte transmembrane protein (OPTN) or peripheral myelin protein 22-alpha (PMP22α), is a member of the claudin family of transmembrane proteins that play critical roles in forming tight junctions. These junctions regulate paracellular permeability and maintain cell polarity in epithelial and endothelial tissues. Structurally, CLDN11 consists of four transmembrane domains, two extracellular loops, and intracellular N- and C-termini. Its extracellular loops mediate homotypic or heterotypic interactions with adjacent claudins to create selective barriers.
CLDN11 is particularly notable for its expression in myelinating cells, including oligodendrocytes in the central nervous system (CNS) and Sertoli cells in the testes. In the CNS, it is essential for forming the tight junctions of the myelin sheath, ensuring efficient nerve conduction. In the testes, it contributes to the blood-testis barrier, safeguarding germ cell development. Dysregulation of CLDN11 has been linked to neurological disorders (e.g., multiple sclerosis), male infertility, and certain cancers.
Recombinant CLDN11 protein is engineered using expression systems like *E. coli* or mammalian cells, followed by purification techniques such as affinity chromatography. This protein retains functional domains, enabling studies on its interaction partners, barrier-forming mechanisms, and signaling pathways. Researchers utilize it to investigate tissue-specific tight junction dynamics, screen therapeutic agents targeting claudin-related pathologies, or develop biomarkers for diseases. Its recombinant form offers advantages like high purity, controlled post-translational modifications (in eukaryotic systems), and scalability, making it valuable for both basic research and translational applications, including drug discovery and regenerative medicine.
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