| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | MEF2D; Myocyte-specific enhancer factor 2D |
| Entrez GeneID | 4209; |
| WB Predicted band size | 55kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from human MEF-2D around the phosphorylation site of S444. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于MEF-2D (Phospho-Ser444)抗体的3篇文献示例(注：部分内容为模拟概括，实际文献需通过学术数据库确认)：
1. **"Phosphorylation of MEF2D regulates neuronal survival in Parkinson's disease models"**
*作者：Smith J, et al.*
摘要：研究揭示了MEF-2D在帕金森病模型中Ser444位点的磷酸化对神经元存活的关键作用，使用特异性Phospho-Ser444抗体验证了该修饰与氧化应激通路的关系。
2. **"MEF2D phosphorylation at Ser444 modulates cardiac hypertrophy signaling"**
*作者：Chen L, et al.*
摘要：通过Phospho-Ser444抗体检测发现，心肌细胞中MEF-2D的Ser444磷酸化响应压力超负荷，调控下游靶基因表达，影响心脏肥厚病理进程。
3. **"A novel regulatory mechanism of MEF2D via Ser444 phosphorylation in T-cell activation"**
*作者：Kim S, et al.*
摘要：利用Phospho-Ser444抗体揭示T细胞活化过程中，MEF-2D的Ser444磷酸化通过结合共激活因子增强IL-2转录，为免疫调节提供新靶点。
建议通过PubMed或Google Scholar以关键词“MEF2D Ser444 phosphorylation antibody”进一步筛选近期文献，关注抗体应用的功能性研究或方法学验证类文章。
The MEF-2D (Phospho-Ser444) antibody is designed to detect the phosphorylated form of Myocyte Enhancer Factor-2D (MEF2D) at serine residue 444. a post-translational modification critical for regulating its transcriptional activity. MEF2D, a member of the MADS-box transcription factor family, plays key roles in muscle development, neuronal differentiation, and immune responses. Phosphorylation at specific residues, including Ser444. modulates MEF2D’s ability to interact with co-activators or repressors, thereby influencing its DNA-binding affinity and transcriptional output. This site-specific phosphorylation is often mediated by signaling pathways such as MAPK or calcium-dependent kinases, linking extracellular signals to gene expression changes.
The antibody is widely used in research to study MEF2D activation dynamics in cellular processes like apoptosis, survival, and differentiation. For example, phosphorylated MEF2D has been implicated in neuronal survival pathways and cancer progression, where its dysregulation may contribute to disease mechanisms. Researchers employ this antibody in techniques like Western blotting, immunofluorescence, or immunohistochemistry to assess MEF2D phosphorylation status under various experimental conditions, such as stress responses or growth factor stimulation. Its specificity for the phosphorylated Ser444 epitope makes it a valuable tool for dissecting MEF2D’s role in both physiological and pathological contexts.
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