| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 1/200-1/1000 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | STMN1; C1orf215; LAP18; OP18; Stathmin; Leukemia-associated phosphoprotein p18; Metablastin; Oncoprotein 18; Op18; Phosphoprotein p19; pp19; Prosolin; Protein Pr22; pp17 |
| Entrez GeneID | 3925; |
| WB Predicted band size | 17kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from human Op18 around the phosphorylation site of S16. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是3篇涉及Op18 (Phospho-Ser16)抗体的参考文献摘要：
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1. **"Phosphorylation of stathmin at Ser16 modulates microtubule dynamics in mitotic cells"**
*Authors: Belmont LD, Mitchison TJ*
摘要：该研究通过Op18 (Stathmin)的Ser16磷酸化特异性抗体，揭示了该位点在有丝分裂过程中对微管稳定性的调控作用，发现磷酸化抑制Stathmin的微管解聚活性，促进纺锤体形成。
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2. **"Op18 phosphorylation is a critical determinant of cell cycle progression in leukemia cells"**
*Authors: Marklund U, Brattsand G, et al.*
摘要：利用Phospho-Ser16抗体检测白血病细胞周期中Op18的动态磷酸化，证明其磷酸化水平与细胞周期G2/M期进程相关，并受CDK1激酶调控。
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3. **"Site-specific phosphorylation of stathmin modulates its interaction with tubulin and cancer cell migration"**
*Authors: Gavet O, Ozon S, et al.*
摘要：通过Ser16磷酸化特异性抗体进行免疫印迹分析，发现该位点的磷酸化减弱Stathmin与微管蛋白的结合能力，从而抑制乳腺癌细胞的迁移和侵袭表型。
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这些文献均使用了针对Op18 Ser16磷酸化位点的抗体进行机制研究，涵盖细胞周期调控、癌症生物学等领域。如需具体实验细节或抗体来源，建议通过PubMed或Google Scholar按标题进一步检索。
The Op18 (Phospho-Ser16) antibody is designed to detect the phosphorylated form of Op18 (Oncoprotein 18), also known as Stathmin 1. at serine residue 16. Op18 is a ubiquitously expressed cytosolic protein that plays a critical role in regulating microtubule dynamics by binding to tubulin and promoting microtubule depolymerization. Its activity is tightly controlled by phosphorylation, particularly during cell cycle progression and stress responses. Phosphorylation at Ser16. along with other residues (e.g., Ser25. Ser38. Ser63), reduces Op18's microtubule-destabilizing activity, stabilizing microtubules during mitosis or differentiation. This modification is mediated by kinases such as protein kinase A (PKA) and calcium/calmodulin-dependent kinase IV (CaMKIV), linking Op18 phosphorylation to signaling pathways involved in cell proliferation, apoptosis, and neuronal function.
The Op18 (Phospho-Ser16) antibody is widely used in research to study the regulatory mechanisms of microtubule dynamics, cell cycle checkpoints, and cancer biology, as Op18 overexpression or dysregulated phosphorylation is associated with tumorigenesis and chemoresistance. It is commonly applied in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess phosphorylation-dependent Op18 activity in cellular or tissue samples. Researchers utilize this antibody to explore how Ser16 phosphorylation impacts cellular processes such as mitotic spindle formation, intracellular transport, and cell motility, providing insights into diseases characterized by microtubule instability or aberrant cell proliferation.
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