Recombinant Human HIST1H3A protein

**Background of HIST1H3A Recombinant Protein**

HIST1H3A, a member of the histone H3 family, encodes a core component of nucleosomes, the fundamental repeating units of chromatin. Histones play a critical role in DNA packaging, gene regulation, and epigenetic inheritance. The HIST1H3A gene, located on chromosome 6. is part of a large histone gene cluster (HIST1) that ensures the tightly regulated expression of replication-dependent histones during the cell cycle. The encoded protein, histone H3.1. is a canonical histone variant predominantly incorporated into chromatin during DNA replication.

Recombinant HIST1H3A protein is produced in vitro using expression systems (e.g., *E. coli* or mammalian cells*) to study its structural and functional roles. It retains post-translational modification (PTM) sites, such as lysine residues susceptible to methylation, acetylation, or phosphorylation, which are pivotal for epigenetic signaling. These modifications regulate chromatin accessibility, transcriptional activity, and cellular processes like DNA repair and chromosome segregation.

Research involving HIST1H3A recombinant protein often focuses on its dysregulation in diseases. For example, somatic mutations in HIST1H3A (e.g., K27M or G34R/V) are linked to aggressive cancers, including pediatric gliomas, altering histone PTM patterns and driving oncogenic gene expression. Additionally, recombinant HIST1H3A serves as a substrate in assays to study enzymes like histone methyltransferases or acetyltransferases, aiding drug discovery for epigenetic therapies. Its use also extends to structural studies, antibody production, and understanding chromatin remodeling mechanisms. Overall, HIST1H3A recombinant protein is a vital tool for unraveling histone biology and its implications in health and disease.

(*Note: Expression systems may vary depending on PTM requirements.)