| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HIST1H3A |
| Uniprot No | P68431 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-136aa |
| 氨基酸序列 | ARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEACEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA |
| 预测分子量 | 18.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HIST1H3A重组蛋白的3篇代表性文献摘要(注：文献为虚拟示例，实际检索需通过数据库验证)：
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1. **文献名称**：*"Expression and purification of recombinant human histone H3.1 (HIST1H3A) for chromatin assembly studies"*
**作者**：Smith J, et al.
**摘要**：本研究描述了一种在大肠杆菌中高效表达和纯化重组人源HIST1H3A蛋白的方法，通过优化密码子使用和纯化步骤获得高纯度蛋白，并验证其在体外核小体重组实验中的功能性应用。
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2. **文献名称**：*"H3K36me2-specific recognition by histone readers requires intact HIST1H3A N-terminal tail"*
**作者**：Li X, et al.
**摘要**：通过构建HIST1H3A重组蛋白及其截短突变体，揭示了其N端尾部对结合甲基化阅读蛋白(如PSIP1)的关键作用，为组蛋白修饰与基因调控的分子机制提供了实验依据。
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3. **文献名称**：*"Site-specific mutagenesis of HIST1H3A reveals oncogenic mutations disrupt chromatin stability"*
**作者**：Wang R, et al.
**摘要**：利用重组HIST1H3A蛋白结合体外染色质组装系统，发现胶质瘤相关突变(如K36M)导致核小体结构异常，阐明了其通过表观遗传失调促进肿瘤发生的机制。
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如需具体文献，建议通过PubMed或Web of Science以关键词“HIST1H3A recombinant protein”或“H3.1 expression”进一步检索。
**Background of HIST1H3A Recombinant Protein**
HIST1H3A, a member of the histone H3 family, encodes a core component of nucleosomes, the fundamental repeating units of chromatin. Histones play a critical role in DNA packaging, gene regulation, and epigenetic inheritance. The HIST1H3A gene, located on chromosome 6. is part of a large histone gene cluster (HIST1) that ensures the tightly regulated expression of replication-dependent histones during the cell cycle. The encoded protein, histone H3.1. is a canonical histone variant predominantly incorporated into chromatin during DNA replication.
Recombinant HIST1H3A protein is produced in vitro using expression systems (e.g., *E. coli* or mammalian cells*) to study its structural and functional roles. It retains post-translational modification (PTM) sites, such as lysine residues susceptible to methylation, acetylation, or phosphorylation, which are pivotal for epigenetic signaling. These modifications regulate chromatin accessibility, transcriptional activity, and cellular processes like DNA repair and chromosome segregation.
Research involving HIST1H3A recombinant protein often focuses on its dysregulation in diseases. For example, somatic mutations in HIST1H3A (e.g., K27M or G34R/V) are linked to aggressive cancers, including pediatric gliomas, altering histone PTM patterns and driving oncogenic gene expression. Additionally, recombinant HIST1H3A serves as a substrate in assays to study enzymes like histone methyltransferases or acetyltransferases, aiding drug discovery for epigenetic therapies. Its use also extends to structural studies, antibody production, and understanding chromatin remodeling mechanisms. Overall, HIST1H3A recombinant protein is a vital tool for unraveling histone biology and its implications in health and disease.
(*Note: Expression systems may vary depending on PTM requirements.)
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