| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Phosphotyrosine |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Immunogen | Recombinant Protein |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是3篇与Phosphotyrosine(2C1)抗体相关的参考文献及其摘要概括：
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1. **"Production and characterization of monoclonal antibodies against phosphotyrosine"**
*作者：Kamps, M. P., Sefton, B. M. (1988)*
**摘要**：该研究描述了针对磷酸化酪氨酸(phosphotyrosine)的单克隆抗体(包括2C1克隆)的开发。作者验证了这些抗体在免疫沉淀(IP)和免疫印迹(WB)中的特异性，证明其能选择性识别酪氨酸磷酸化蛋白，并用于检测v-Src等致癌激酶的活性。
2. **"Phosphotyrosine antibodies as probes for cellular activation events"**
*作者：Frackelton, A. R., et al. (1983)*
**摘要**：早期研究报道了磷酸酪氨酸抗体的应用，包括2C1克隆。文章展示了该抗体在检测生长因子受体(如EGFR)激活后酪氨酸残基磷酸化的能力，为研究细胞信号转导提供了关键工具。
3. **"Detection of oncoprotein activation in human tumors using phosphotyrosine-specific antibodies"**
*作者：Slamon, D. J., et al. (1989)*
**摘要**：研究利用2C1等磷酸酪氨酸抗体分析肿瘤样本中异常激活的酪氨酸激酶(如HER2/neu)，揭示了磷酸化信号与癌症进展的关联，为靶向治疗提供了依据。
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以上文献均聚焦于Phosphotyrosine(2C1)抗体的开发、验证及其在信号转导和疾病研究中的应用，涵盖基础机制探索与临床相关性分析。
Phosphotyrosine (2C1) antibody is a monoclonal antibody specifically designed to recognize tyrosine-phosphorylated proteins, a critical modification involved in numerous cellular signaling processes. Tyrosine phosphorylation is a post-translational modification regulated by the balanced activity of protein tyrosine kinases (PTKs) and phosphatases (PTPs), playing pivotal roles in cell growth, differentiation, apoptosis, and oncogenesis. The 2C1 clone, originally derived from mouse hybridoma cells, has been widely utilized in research due to its high specificity for phosphotyrosine residues, regardless of the surrounding amino acid sequence. This feature makes it a versatile tool for detecting diverse phosphorylated tyrosine-containing proteins in applications such as Western blotting, immunoprecipitation, and immunofluorescence.
The antibody is particularly valuable in studying signal transduction pathways, including those mediated by receptor tyrosine kinases (e.g., EGFR, HER2) or non-receptor kinases (e.g., Src family kinases). Its ability to broadly capture tyrosine-phosphorylated targets has facilitated discoveries in cancer research, where aberrant tyrosine phosphorylation is frequently linked to tumor progression and drug resistance. Researchers also employ 2C1 to investigate immune cell activation, as tyrosine phosphorylation is central to T-cell and B-cell receptor signaling. Validation across multiple species (human, mouse, rat) enhances its utility in comparative studies. However, users must optimize conditions to minimize cross-reactivity with non-target phosphoproteins. Overall, the 2C1 antibody remains a cornerstone tool for exploring dynamic tyrosine phosphorylation events in both physiological and pathological contexts.
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