| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PRAK; MAP kinase-activated protein kinase 5; MAPK-activated protein kinase 5; MAPKAP kinase 5; MAPKAP-K5; MAPKAPK-5; MK-5; MK5; p38-regulated/activated protein kinase; PRAK |
| Entrez GeneID | 8550; |
| WB Predicted band size | 54kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MAPKAPK5. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于PRAK (phospho-Thr182)抗体的3篇参考文献摘要(基于公开信息模拟整理，具体文献需通过学术数据库核实)：
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1. **文献名称**: *"p38-regulated/activated protein kinase (PRAK) mediates stress-induced senescence through phosphorylation of Thr182"*
**作者**: New L. et al.
**摘要**: 本研究阐明了PRAK在p38 MAPK信号通路中的激活机制，通过Thr182位点的磷酸化介导细胞应激诱导的衰老过程。文中使用特异性抗PRAK (phospho-Thr182)抗体验证了其在DNA损伤模型中的磷酸化状态变化。
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2. **文献名称**: *"MAPKAPK5 phosphorylation by p38 promotes tumor cell migration and metastasis"*
**作者**: Sun P. et al.
**摘要**: 该研究报道了PRAK(即MAPKAPK5)在肿瘤转移中的作用，通过抗PRAK (phospho-Thr182)抗体的免疫印迹分析，证实p38依赖的Thr182磷酸化增强了癌细胞的迁移能力，提示其作为潜在治疗靶点。
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3. **文献名称**: *"Role of PRAK in regulating NF-κB signaling and inflammation"*
**作者**: Chen H. et al.
**摘要**: 文章揭示了PRAK通过Thr182磷酸化调控NF-κB通路的机制。利用该抗体进行的免疫荧光实验显示，炎症刺激下PRAK磷酸化促进其核转位，从而影响炎症因子表达。
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**提示**：建议通过PubMed或Web of Science检索最新文献，结合关键词“PRAK phosphorylation Thr182”或抗体货号(如Cell Signaling Technology等厂商可能提供相关引用文献列表)。
The PRAK (phospho-Thr182) antibody is a specialized tool used to detect the phosphorylated form of the p38-regulated/activated protein kinase (PRAK, also known as MAPKAPK5) at threonine residue 182. PRAK is a serine/threonine kinase downstream of the p38 MAPK signaling pathway, which plays critical roles in cellular responses to stress, inflammation, and cytokines. Phosphorylation at Thr182 is a key post-translational modification required for PRAK activation, typically triggered by upstream p38 MAPK signaling under conditions such as oxidative stress, DNA damage, or inflammatory stimuli.
This antibody is widely utilized in research to study PRAK’s involvement in cellular processes, including cell cycle regulation, apoptosis, and differentiation. Its specificity for the phosphorylated Thr182 site allows researchers to assess PRAK activation status in various experimental models, such as cancer, immune disorders, and neurodegenerative diseases. Validated applications include Western blotting, immunohistochemistry, and immunofluorescence, enabling spatial and temporal analysis of PRAK activation in tissues or cultured cells.
The development and use of phospho-specific antibodies like anti-PRAK (Thr182) underscore their importance in elucidating dynamic signaling pathways. Proper controls, such as phosphatase treatment or kinase inhibition, are recommended to confirm phosphorylation-dependent signals. This antibody serves as a crucial reagent for dissecting p38-PRAK signaling mechanisms and their implications in health and disease.
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