关键信息
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基因名
KCNJ12
- 应用
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别名
KCNJ12; IRK2; KCNJN1; ATP-sensitive inward rectifier potassium channel 12; Inward rectifier K(+ channel Kir2.2; IRK-2; Inward rectifier K(+ channel Kir2.2v; Potassium channel. inwardly rectifying subfamily J member 12
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q14500
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表达区间
1-433aa
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氨基酸序列
MTAASRANPYSIVSSEEDGLHLVTMSGANGFGNGKVHTRRRCRNRFVKKNGQCNIEFANMDEKSQRYLADMFTTCVDIRWRYMLLIFSLAFLASWLLFGIIFWVIAVAHGDLEPAEGRGRTPCVMQVHGFMAAFLFSIETQTTIGYGLRCVTEECPVAVFMVVAQSIVGCIIDSFMIGAIMAKMARPKKRAQTLLFSHNAVVALRDGKLCLMWRVGNLRKSHIVEAHVRAQLIKPRVTEEGEYIPLDQIDIDVGFDKGLDRIFLVSPITILHEIDEASPLFGISRQDLETDDFEIVVILEGMVEATAMTTQARSSYLANEILWGHRFEPVLFEEKNQYKIDYSHFHKTYEVPSTPRCSAKDLVENKFLLPSANSFCYENELAFLSRDEEDEADGDQDGRSRDGLSPQARHDFDRLQAGGGVLEQRPYRRESEI
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分子量
73.37 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
KCNJ12, also known as the Kir2.1 potassium channel, is a member of the inwardly rectifying potassium channel family, which plays a crucial role in maintaining the resting membrane potential and regulating excitability in various cell types, including neurons and cardiomyocytes. Mutations in the KCNJ12 gene have been associated with arrhythmias and other cardiac disorders, highlighting its significant role in physiological and pathological processes. The recombinant expression of KCNJ12 protein is essential for elucidating its functional properties and understanding its role in ion transport and electrical signaling. Researchers utilize various expression systems, such as mammalian cells or yeast, to produce functional KCNJ12 channels for biophysical and pharmacological studies. The characterization of KCNJ12 through electrophysiological techniques helps to reveal its gating mechanisms, ion selectivity, and response to pharmacological agents. Additionally, the study of KCNJ12 is vital for developing targeted therapies for conditions related to potassium channel dysfunction. Understanding the structure-function relationship of KCNJ12 can provide insights into the development of drugs aimed at modulating its activity, thereby offering potential therapeutic strategies for managing cardiac and neurological disorders linked to this channel. Given the increasing recognition of the importance of potassium channels in health and disease, research on KCNJ12 continues to be a significant area of focus in molecular physiology and pharmacology.
