| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CRABP2 |
| Uniprot No | P29373 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-138aa |
| 氨基酸序列 | MPNFSGNWKI IRSENFEELL KVLGVNVMLR KIAVAAASKP AVEIKQEGDT FYIKTSTTVR TTEINFKVGE EFEEQTVDGR PCKSLVKWES ENKMVCEQKL LKGEGPKTSW TRELTNDGEL ILTMTADDVV CTRVYVRE |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Crystal structure of recombinant human cellular retinoic acid-binding protein II (CRABP2) complexed with all-trans-retinoic acid"**
*Authors: Kleywegt GJ, Jones TA*
摘要：通过X射线晶体学解析了重组人CRABP2与全反式视黄酸结合的复合物结构，揭示了配体结合口袋的构象变化及关键氨基酸残基的相互作用机制。
2. **"CRABP2 enhances retinoic acid signaling by facilitating nuclear transport of retinoic acid"**
*Authors: Dong D, Noy N*
摘要：研究利用重组CRABP2蛋白验证其作为视黄酸转运载体的功能，证明其通过促进视黄酸向细胞核的转运，增强下游基因转录活性。
3. **"Recombinant CRABP2-FABP5 fusion protein as a potential drug delivery carrier for cancer therapy"**
*Authors: Budhu AS, Napoli JL*
摘要：构建CRABP2与FABP5的融合重组蛋白，验证其结合疏水性抗癌药物的能力，并展示其在体外增强药物靶向癌细胞的效果。
4. **"CRABP2 modulates MMP2 expression to promote cancer cell invasion via non-genomic retinoic acid signaling"**
*Authors: Sessler RJ, Noy N*
摘要：通过重组CRABP2蛋白实验，发现其通过非基因组信号通路调控基质金属蛋白酶MMP2的表达，进而促进癌细胞迁移和侵袭。
Cellular retinoic acid-binding protein 2 (CRABP2) is a small cytosolic protein belonging to the lipid-binding protein family, primarily involved in mediating the intracellular transport and metabolism of retinoic acid (RA), the active derivative of vitamin A. It facilitates the delivery of RA to nuclear retinoic acid receptors (RARs), enabling RA-regulated gene expression critical for cellular differentiation, proliferation, and embryonic development. Unlike its homolog CRABP1. CRABP2 is widely expressed in adult tissues and exhibits a stronger affinity for RA, positioning it as a key modulator of RA signaling pathways.
Recombinant CRABP2 protein is engineered through molecular cloning techniques, typically expressed in bacterial (e.g., *E. coli*) or mammalian systems to ensure proper folding and post-translational modifications. Purification methods often involve affinity chromatography tags (e.g., His-tag) for high yield and purity. Structurally, the protein features a conserved β-barrel domain with a hydrophobic ligand-binding pocket, enabling selective RA binding and shielding it from non-specific enzymatic degradation.
Research on recombinant CRABP2 has illuminated its roles in cancer progression, skin homeostasis, and neurogenesis. Overexpression of CRABP2 has been linked to enhanced tumor invasiveness in certain cancers (e.g., breast, ovarian), while its interaction with RA pathways underscores therapeutic potential in dermatology (e.g., psoriasis, acne) and oncology (e.g., acute promyelocytic leukemia). Additionally, recombinant CRABP2 serves as a tool to study RA metabolism, receptor crosstalk, and ligand-mediated gene regulation, offering insights into drug design targeting RA-dependent diseases. Its biochemical stability and functional versatility make it a valuable reagent for both basic research and biopharmaceutical applications.
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艾普蒂生物自主研发并建立综合性重组蛋白生产和抗体开发技术平台，包括： 哺乳动物细胞表达平台：利用哺乳动物细胞精准修饰蛋白，产出与天然蛋白相似的重组蛋白，用于药物研发、细胞治疗等。 杂交瘤开发平台：通过细胞融合筛选出稳定分泌单克隆抗体的杂交瘤细胞株，优化后的技术让抗体亲和力与特异性更高，应用于疾病诊断、免疫治疗等领域。 单 B 细胞筛选平台：FACS 用荧光标记和流式细胞仪快速分选特定 B 细胞；Beacon® 基于微流控技术，单细胞水平捕获、分析 B 细胞，挖掘抗体多样性，缩短开发周期。 凭借这些平台，艾普蒂生物为客户提供优质试剂和专业 CRO 技术服务，推动生物科技发展。
艾普蒂生物在重组蛋白和天然蛋白开发领域经验十分丰富，拥有超过 2 万种重组蛋白的开发案例。在四大重组蛋白表达平台的运用上，艾普蒂生物不仅经验老到，还积累了详实的成功案例。针对客户的工业化生产需求，我们能够定制并优化实验方案。通过小试探索、工艺放大以及条件优化等环节，对重组蛋白基因序列进行优化，全面探索多种条件，精准找出最契合客户需求的生产方法。 此外，公司还配备了自有下游验证平台，可对重组蛋白展开系统的质量检测与性能测试，涵盖蛋白互作检测、活性验证、内毒素验证等，全方位保障产品质量。 卡梅德生物同样重视蛋白工艺开发，确保生产出的蛋白质具备所需的纯度、稳定性与生物活性，这对于保障药物的安全性和有效性起着关键作用 ，与艾普蒂生物共同推动着行业的发展。
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