| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KIFBP |
| Uniprot No | Q96EK5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-621aa |
| 氨基酸序列 | MANVPWAEVCEKFQAALALSRVELHKNPEKEPYKSKYSARALLEEVKALLGPAPEDEDERPEAEDGPGAGDHALGLPAEVVEPEGPVAQRAVRLAVIEFHLGVNHIDTEELSAGEEHLVKCLRLLRRYRLSHDCISLCIQAQNNLGILWSEREEIETAQAYLESSEALYNQYMKEVGSPPLDPTERFLPEEEKLTEQERSKRFEKVYTHNLYYLAQVYQHLEMFEKAAHYCHSTLKRQLEHNAYHPIEWAINAATLSQFYINKLCFMEARHCLSAANVIFGQTGKISATEDTPEAEGEVPELYHQRKGEIARCWIKYCLTLMQNAQLSMQDNIGELDLDKQSELRALRKKELDEEESIRKKAVQFGTGELCDAISAVEEKVSYLRPLDFEEARELFLLGQHYVFEAKEFFQIDGYVTDHIEVVQDHSALFKVLAFFETDMERRCKMHKRRIAMLEPLTVDLNPQYYLLVNRQIQFEIAHAYYDMMDLKVAIADRLRDPDSHIVKKINNLNKSALKYYQLFLDSLRDPNKVFPEHIGEDVLRPAMLAKFRVARLYGKIITADPKKELENLATSLEHYKFIVDYCEKHPEAAQEIEVELELSKEMVSLLPTKMERFRTKMALT |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KIFBP重组蛋白的3篇参考文献示例(注：文献为模拟示例，仅供参考)：
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1. **文献名称**：*Recombinant Expression and Functional Analysis of KIFBP in Cytoskeletal Regulation*
**作者**：Chen L, et al.
**摘要**：研究利用大肠杆菌系统成功表达并纯化重组KIFBP蛋白，验证其与驱动蛋白家族成员KIF5B的相互作用，发现KIFBP通过调控微管网络稳定性影响细胞迁移。
2. **文献名称**：*KIFBP Recombinant Protein as a Novel Therapeutic Target in Neurodegenerative Disorders*
**作者**：Wang H, et al.
**摘要**：通过昆虫细胞表达系统制备高纯度KIFBP重组蛋白，揭示其在神经元轴突运输中的功能缺失与阿尔茨海默病模型中的tau蛋白异常聚集相关。
3. **文献名称**：*Structural Characterization of KIFBP Using Recombinant Protein Technology*
**作者**：Kimura T, et al.
**摘要**：结合冷冻电镜技术分析重组KIFBP的三维结构，阐明其结合Kinesin-1的分子机制，为开发抑制肿瘤转移的小分子化合物提供结构基础。
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**说明**：以上文献为模拟生成，实际研究中请通过学术数据库(如PubMed、Web of Science)检索真实发表的论文。建议使用关键词“KIFBP recombinant protein”或“KIF1BP”进行精确查找。若研究领域较新，可扩展检索相关驱动蛋白(Kinesin)结合蛋白的重组表达文献。
Kinesin family binding protein (KIFBP), also known as KIF1BP, is a cytosolic protein that interacts with members of the kinesin superfamily, a group of molecular motors critical for intracellular transport, mitosis, and organelle positioning. Initially identified through yeast two-hybrid screening, KIFBP is evolutionarily conserved and ubiquitously expressed, with particularly high levels in neural tissues. It plays a regulatory role in kinesin-mediated processes by modulating motor protein activity, cargo binding, or microtubule interactions. Structural studies reveal multiple coiled-coil domains that facilitate protein-protein interactions.
The recombinant KIFBP protein, typically produced in E. coli or mammalian expression systems, enables functional characterization and interaction studies. Researchers employ affinity-tagged versions (e.g., His-tag, GST-tag) for purification and in vitro assays. Recombinant KIFBP has been instrumental in identifying its binding partners, including KIF1A and KIF1Bβ, which are involved in neuronal transport. Dysregulation of KIFBP has been implicated in neurodevelopmental disorders and cancer progression, making it a potential therapeutic target. Current applications span from elucidating axonal transport mechanisms to screening small molecule inhibitors. Challenges remain in fully mapping its post-translational modifications and tissue-specific isoforms, which recombinant protein systems help address through controlled expression studies.
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