关键信息
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基因名
HSD17b12
- 应用
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别名
HSD17b12；SSC1；Very long chain fatty acid elongase 1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q53GQ0
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表达区间
1-312aa
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氨基酸序列
MESALPAAGFLYWVGAGTVAYLALRISYSLFTALRVWGVGNEAGVGPGLGEWAVVTGSTDGIGKSYAEELAKHGMKVVLISRSKDKLDQVSSEIKEKFKVETRTIAVDFASEDIYDKIKTGLAGLEIGILVNNVGMSYEYPEYFLDVPDLDNVIKKMININILSVCKMTQLVLPGMVERSKGAILNISSGSGMLPVPLLTIYSATKTFVDFFSQCLHEEYRSKGVFVQSVLPYFVATKLAKIRKPTLDKPSPETFVKSAIKTVGLQSRTNGYLIHALMGSIISNLPSWIYLKIVMNMNKSTRAHYLKKTKKN
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分子量
34.3 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
HSD17B12 (17β-hydroxysteroid dehydrogenase type 12) is an enzyme involved in the biosynthesis of steroid hormones and lipids, playing a critical role in the metabolism of fatty acids and cholesterol. Recent studies have highlighted its importance in various physiological processes, including spermatogenesis and energy metabolism, as well as its potential implications in metabolic disorders and reproductive health. The enzyme catalyzes the conversion of various 17-ketosteroids to their corresponding 17-hydroxysteroids, which are essential in the regulation of anabolic and catabolic pathways. Given its pivotal function, HSD17B12 has emerged as a potential therapeutic target for diseases related to steroid hormone imbalance and lipid metabolism, such as obesity and infertility. Research on recombinant HSD17B12 protein enables scientists to further elucidate its enzymatic properties, substrate specificity, and interactions with other metabolic pathways. By generating recombinant forms of HSD17B12, researchers aim to develop inhibitors or activators that could modulate its activity, providing insights into novel therapeutic avenues and expanding our understanding of steroid biosynthesis and its regulatory mechanisms in human health and disease. The exploration of HSD17B12's role in these biological contexts underscores its significance in both basic research and potential clinical applications.
