关键信息
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基因名
UGT2B17
- 应用
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别名
UGT2B17；UDP-glucuronosyltransferase 2B17
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O75795
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表达区间
24-530aa
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氨基酸序列
GKVLVWPTEYSHWINMKTILEELVQRGHEVIVLTSSASILVNASKSSAIKLEVYPTSLTKNDLEDFFMKMFDRWTYSISKNTFWSYFSQLQELCWEYSDYNIKLCEDAVLNKKLMRKLQESKFDVLLADAVNPCGELLAELLNIPFLYSLRFSVGYTVEKNGGGFLFPPSYVPVVMSELSDQMIFMERIKNMIYMLYFDFWFQAYDLKKWDQFYSEVLGRPTTLFETMGKAEMWLIRTYWDFEFPRPFLPNVDFVGGLHCKPAKPLPKEMEEFVQSSGENGIVVFSLGSMISNMSEESANMIASALAQIPQKVLWRFDGKKPNTLGSNTRLYKWLPQNDLLGHPKTKAFITHGGTNGIYEAIYHGIPMVGIPLFADQHDNIAHMKAKGAALSVDIRTMSSRDLLNALKSVINDPIYKENIMKLSRIHHDQPVKPLDRAVFWIEFVMRHKGAKHLRVAAHNLTWIQYHSLDVIAFLLACVATMIFMITKCCLFCFRKLAKTGKKKKRD
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分子量
64.5 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
UGT2B17 is a member of the UDP-glucuronosyltransferase (UGT) family, which plays a crucial role in the metabolic processing and detoxification of various endogenous and exogenous compounds. Specifically, UGT2B17 is responsible for the glucuronidation of many steroid hormones and drugs, influencing their pharmacokinetics and pharmacodynamics. Variations in UGT2B17 gene expression can significantly impact individual responses to medications, leading to variations in drug efficacy and toxicity. Research has shown that this enzyme is involved in the metabolism of substances like testosterone and certain therapeutic agents, making it important in hormone regulation and drug metabolism. Understanding the structure and function of UGT2B17 is essential for pharmacogenomics, as genetic polymorphisms may contribute to interindividual variability in drug response. Furthermore, studying the recombinant protein form of UGT2B17 allows researchers to investigate the enzyme's catalytic properties and interactions with various substrates in vitro. This research can enhance our understanding of its role in drug metabolism and inform personalized medicine approaches, particularly in the context of optimizing drug therapy and minimizing adverse effects in patients. The production and characterization of recombinant UGT2B17 serve as pivotal steps toward elucidating the enzyme's functional dynamics and its potential implications in clinical settings.
