关键信息
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基因名
nuc1
- 应用
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别名
nuc1；NR1C2；PPARB；Peroxisome proliferator-activated receptor delta
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q03181
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表达区间
165-441aa
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氨基酸序列
GSQYNPQVADLKAFSKHIYNAYLKNFNMTKKKARSILTGKASHTAPFVIHDIETLWQAEKGLVWKQLVNGLPPYKEISVHVFYRCQCTTVETVRELTEFAKSIPSFSSLFLNDQVTLLKYGVHEAIFAMLASIVNKDGLLVANGSGFVTREFLRSLRKPFSDIIEPKFEFAVKFNALELDDSDLALFIAAIILCGDRPGLMNVPRVEAIQDTILRALEFHLQANHPDAQYLFPKLLQKMADLRQLVTEHAQMMQRIKKTETETSLHPLLQEIYKDMY
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分子量
49.8 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
Nuc1, a recombinant protein derived from the nuclease enzyme family, plays a crucial role in various biological processes, particularly in DNA metabolism and cellular responses to stress. Its study has gained significant attention due to its potential applications in molecular biology and biotechnology. Understanding the structure and functional mechanisms of Nuc1 is essential for its utilization in gene editing, recombinant DNA technology, and therapeutic interventions. Recent advances in protein engineering and expression systems have facilitated the production of Nuc1 in various host organisms, enabling researchers to explore its enzymatic activity and interaction with nucleic acids. Furthermore, Nuc1 has been implicated in studies of apoptosis and cellular signaling, making it a valuable target for therapeutic strategies in diseases characterized by dysregulated cell death. Ongoing research aims to elucidate the biochemical pathways involving Nuc1, which may lead to novel insights in both basic and applied sciences, including cancer research and the development of innovative biopharmaceuticals. The ongoing exploration of Nuc1 underscores its relevance in advancing our understanding of molecular mechanisms and enhancing biotechnological applications.
