| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NCSTN |
| Uniprot No | Q92542-1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-669aa |
| 氨基酸序列 | MATAGGGSGA DPGSRGLLRL LSFCVLLAGL CRGNSVERKI YIPLNKTAPC VRLLNATHQI GCQSSISGDT GVIHVVEKEE DLQWVLTDGP NPPYMVLLES KHFTRDLMEK LKGRTSRIAG LAVSLTKPSP ASGFSPSVQC PNDGFGVYSN SYGPEFAHCR EIQWNSLGNG LAYEDFSFPI FLLEDENETK VIKQCYQDHN LSQNGSAPTF PLCAMQLFSH MHAVISTATC MRRSSIQSTF SINPEIVCDP LSDYNVWSML KPINTTGTLK PDDRVVVAAT RLDSRSFFWN VAPGAESAVA SFVTQLAAAE ALQKAPDVTT LPRNVMFVFF QGETFDYIGS SRMVYDMEKG KFPVQLENVD SFVELGQVAL RTSLELWMHT DPVSQKNESV RNQVEDLLAT LEKSGAGVPA VILRRPNQSQ PLPPSSLQRF LRARNISGVV LADHSGAFHN KYYQSIYDTA ENINVSYPEW LSPEEDLNFV TDTAKALADV ATVLGRALYE LAGGTNFSDT VQADPQTVTR LLYGFLIKAN NSWFQSILRQ DLRSYLGDGP LQHYIAVSSP TNTTYVVQYA LANLTGTVVN LTREQCQDPS KVPSENKDLY EYSWVQGPLH SNETDRLPRC VRSTARLARA LSPAFELSQW SSTEYSTWTE SRWKDIRARI FLIASKELE |
| 预测分子量 | 72 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NCSTN重组蛋白的3篇参考文献，涵盖结构、功能及表达研究：
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1. **文献名称**：*Nicastrin functions as a γ-secretase-substrate receptor*
**作者**：Shah S, Lee SF, Tabuchi K, et al.
**摘要**：该研究通过重组NCSTN蛋白与γ-分泌酶复合体其他组分的共表达，揭示了NCSTN作为底物受体在淀粉样前体蛋白(APP)切割中的关键作用，并证明其通过N端结构域识别底物。
2. **文献名称**：*Reconstitution of γ-secretase activity demonstrates roles of nicastrin in substrate recognition*
**作者**：Edbauer D, Winkler E, Haass C, et al.
**摘要**：利用重组NCSTN、早老素(presenilin)等蛋白重建γ-分泌酶活性，发现NCSTN通过调控底物进入酶活性中心，影响β-淀粉样蛋白(Aβ)的生成，为阿尔茨海默病机制提供证据。
3. **文献名称**：*Expression and purification of functional human nicastrin in Drosophila S2 cells*
**作者**：Li Y, Lu SH, Tsai CJ.
**摘要**：报道了一种在果蝇S2细胞中高效表达重组人源NCSTN的方法，通过亲和层析纯化获得活性蛋白，并验证其与γ-分泌酶复合体的结合能力，为体外研究提供可靠工具。
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以上文献涵盖NCSTN的功能机制、复合体组装及重组表达方法，适用于相关生化与疾病研究。如需全文链接或补充信息，可进一步提供具体需求。
NCSTN (nicastrin) is a key component of the gamma-secretase complex, a multisubunit protease critical for intramembrane cleavage of various type I transmembrane proteins. Initially identified as a regulator of Notch signaling, NCSTN plays a pivotal role in the proteolytic processing of amyloid precursor protein (APP), generating amyloid-beta (Aβ) peptides implicated in Alzheimer’s disease pathogenesis. As a large, single-pass transmembrane glycoprotein, NCSTN facilitates substrate recognition and stabilization within the gamma-secretase complex, which also includes presenilin, PEN-2. and APH-1.
Recombinant NCSTN proteins are engineered to study gamma-secretase function, disease mechanisms, and therapeutic interventions. Produced via heterologous expression systems (e.g., mammalian, insect, or bacterial cells), these purified proteins retain structural and functional properties for in vitro assays, structural studies, or antibody development. Researchers leverage recombinant NCSTN to investigate its role in substrate specificity, enzymatic activity modulation, and interactions with inhibitors targeting Aβ production in Alzheimer’s research.
Beyond neurodegeneration, NCSTR重组蛋白 also aids in exploring its involvement in cancer (via Notch signaling), immune regulation, and developmental biology. Mutations in NCSTN are linked to familial acne inversa, highlighting its broader pathophysiological relevance. The development of recombinant NCSTN has advanced drug discovery pipelines, enabling high-throughput screening for gamma-secretase modulators while minimizing off-target effects. Its application extends to diagnostic tools for detecting gamma-secretase dysfunction and biomarker studies in age-related disorders.
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