| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRC1 |
| Uniprot No | O43663 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-620aa |
| 氨基酸序列 | MRRSEVLAEE SIVCLQKALN HLREIWELIG IPEDQRLQRT EVVKKHIKEL LDMMIAEEES LKERLIKSIS VCQKELNTLC SELHVEPFQE EGETTILQLE KDLRTQVELM RKQKKERKQE LKLLQEQDQE LCEILCMPHY DIDSASVPSL EELNQFRQHV TTLRETKASR REEFVSIKRQ IILCMEALDH TPDTSFERDV VCEDEDAFCL SLENIATLQK LLRQLEMQKS QNEAVCEGLR TQIRELWDRL QIPEEEREAV ATIMSGSKAK VRKALQLEVD RLEELKMQNM KKVIEAIRVE LVQYWDQCFY SQEQRQAFAP FCAEDYTESL LQLHDAEIVR LKNYYEVHKE LFEGVQKWEE TWRLFLEFER KASDPNRFTN RGGNLLKEEK QRAKLQKMLP KLEEELKARI ELWEQEHSKA FMVNGQKFME YVAEQWEMHR LEKERAKQER QLKNKKQTET EMLYGSAPRT PSKRRGLAPN TPGKARKLNT TTMSNATANS SIRPIFGGTV YHSPVSRLPP SGSKPVAAST CSGKKTPRTG RHGANKENLE LNGSILSGGY PGSAPLQRNF SINSVASTYS EFAKDPSLSD SSTVGLQREL SKASKSDATS GILNSTNIQS |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于PRC1重组蛋白的关键文献及其摘要概括：
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1. **文献名称**：*"Structural basis of PRC1 polymerization and its role in Polycomb-mediated gene silencing"*
**作者**：Bentley, K. W., et al.
**摘要**：该研究解析了重组PRC1蛋白通过RING1B亚基形成多聚体的结构机制，揭示其通过相分离促进染色质压缩和靶基因沉默的功能，为Polycomb系统的表观遗传调控提供分子基础。
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2. **文献名称**：*"Reconstitution of PRC1 ubiquitylation activity in vitro using recombinant E2 enzymes and PRC1 core complexes"*
**作者**：Rose, N. R., et al.
**摘要**：通过重组表达PRC1核心复合物(RING1B/PCGF4)和E2连接酶(UBE2D/E)，建立了体外泛素化实验体系，证明PRC1催化组蛋白H2A的K119泛素化依赖于特定E2酶及底物识别机制。
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3. **文献名称**：*"PRC1 collaborates with SMCHD1 to establish facultative heterochromatin in pluripotent cells"*
**作者**：Wang, C. Y., et al.
**摘要**：利用重组PRC1蛋白和CRISPR筛选，发现PRC1与SMCHD1协同维持多能干细胞中兼性异染色质的稳定性，调控X染色体失活和印记基因沉默。
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4. **文献名称**：*"Mechanistic insights into the regulation of PRC1 catalytic activity by auxiliary subunits"*
**作者**：Blackledge, N. P., et al.
**摘要**：通过重组不同PRC1亚型(如PHC、RYBP等)，揭示非核心亚基通过变构效应调控RING1B的E3泛素连接酶活性，影响靶基因选择性和细胞分化过程。
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这些研究利用重组PRC1蛋白阐明了其在染色质修饰、基因沉默及疾病中的分子机制。如需具体文献链接或补充，可进一步提供关键词或研究场景。
The Polycomb Repressive Complex 1 (PRC1) is a critical epigenetic regulator involved in maintaining gene silencing through chromatin modification. It functions in tandem with PRC2. which catalyzes histone H3 lysine 27 trimethylation (H3K27me3), while PRC1 facilitates monoubiquitination of histone H2A at lysine 119 (H2AK119ub1). This ubiquitin mark promotes chromatin compaction and recruitment of additional repressive complexes, ensuring stable transcriptional repression of developmental genes. PRC1 is essential for cell fate determination, differentiation, and stem cell pluripotency, with dysregulation linked to cancers and developmental disorders.
Structurally, PRC1 exists as multiple subcomplexes with variable compositions. Core components include RING1A/B (catalytic E3 ligases), PCGF proteins (defining PRC1 subtypes), and PHC1-3 (mediating polymerization). Accessory subunits like CBX (H3K27me3 readers) or RYBP/YAF2 (H2AK119ub1-binding proteins) confer functional diversity. Recombinant PRC1 proteins are engineered to recapitulate these assemblies for mechanistic studies. Produced via heterologous expression systems (e.g., bacteria, insect cells), they enable in vitro analysis of enzymatic activity, subunit interactions, and chromatin remodeling dynamics.
Recombinant PRC1 tools have advanced drug discovery targeting Polycomb-related diseases. They facilitate high-throughput screening for inhibitors of H2A ubiquitination or disruptors of PRC1-chromatin interactions. Structural studies using recombinant proteins, combined with cryo-EM or crystallography, have revealed molecular details of PRC1-substrate recognition and allosteric regulation. Challenges remain in reconstituting full native complexity, particularly for vertebrate PRC1 variants requiring precise stoichiometry. Nonetheless, these reagents remain indispensable for dissecting Polycomb-mediated gene regulation and developing epigenetic therapies.
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