关键信息
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基因名
MED7
- 应用
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别名
MED7;ARC34;CRSP9;Mediator of RNA polymerase II transcription subunit 7
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O43513
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表达区间
1-233aa
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氨基酸序列
MGSSHHHHHH SSGLVPRGSH MGSMGEPQQV SALPPPPMQY IKEYTDENIQ EGLAPKPPPP IKDSYMMFGN QFQCDDLIIR PLESQGIERL HPMQFDHKKE LRKLNMSILI NFLDLLDILI RSPGSIKREE KLEDLKLLFV HVHHLINEYR PHQARETLRV MMEVQKRQRL ETAERFQKHL ERVIEMIQNC LASLPDDLPH SEAGMRVKTE PMDADDSNNC TGQNEHQREN SGHRRDQIIE KDAALCVLID EMNERP
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分子量
30 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
MED7 is a key component of the Mediator complex, which plays a crucial role in the regulation of transcription in eukaryotic cells. The Mediator serves as a bridge between gene-specific transcription factors and the RNA polymerase II enzyme, facilitating the precise control of gene expression. Understanding the structure and function of MED7 is vital, as it is implicated in various biological processes and diseases, including cancer and metabolic disorders. Recent studies have highlighted the potential of MED7 as a therapeutic target, emphasizing the need for its detailed characterization. Recombinant MED7 protein production allows researchers to investigate its molecular interactions, post-translational modifications, and functional dynamics in cellular contexts. By elucidating the role of MED7 in the Mediator complex, researchers aim to uncover new insights into the transcriptional regulation mechanisms, paving the way for novel therapeutic strategies. The development of methodologies for the successful expression and purification of recombinant MED7 protein is a critical step to advance the understanding of its biological significance, facilitating both basic research and potential clinical applications.
