| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PIANP |
| Uniprot No | Q8IYJ0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-282aa |
| 氨基酸序列 | MESRMWPALLLSHLLPLWPLLLLPLPPPAQGSSSSPRTPPAPARPPCARGGPSAPRHVCVWERAPPPSRSPRVPRSRRQVLPGTAPPATPSGFEEGPPSSQYPWAIVWGPTVSREDGGDPNSANPGFLDYGFAAPHGLATPHPNSDSMRGDGDGLILGEAPATLRPFLFGGRGEGVDPQLYVTITISIIIVLVATGIIFKFCWDRSQKRRRPSGQQGALRQEESQQPLTDLSPAGVTVLGAFGDSPTPTPDHEEPRGGPRPGMPHPKGAPAFQLNRIPLV |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PIANP重组蛋白的参考文献示例(注：以下内容为模拟虚构，实际文献需根据真实数据库检索)：
1. **文献名称**：*"Recombinant PIANP promotes neuronal differentiation via EGFR signaling"*
**作者**：Chen Y, et al.
**摘要**：研究通过重组PIANP蛋白处理神经干细胞，发现其通过激活EGFR通路促进神经元分化，为神经再生治疗提供潜在靶点。
2. **文献名称**：*"Expression and functional analysis of PIANP in inflammatory responses"*
**作者**：Wang R, et al.
**摘要**：报道重组PIANP蛋白在巨噬细胞中的表达，证明其通过抑制NF-κB通路减轻炎症反应，提示其抗炎应用潜力。
3. **文献名称**：*"Structural insights into PIANP and its interaction with cellular prion protein"*
**作者**：Suzuki T, et al.
**摘要**：解析重组PIANP蛋白的晶体结构，揭示其与朊病毒蛋白(PrP)的结合位点，探讨二者在神经退行性疾病中的关联。
4. **文献名称**：*"PIANP as a biomarker in cancer: Recombinant protein-based detection"*
**作者**：Li X, et al.
**摘要**：开发基于重组PIANP蛋白的ELISA检测方法，发现其在多种癌症患者血清中高表达，或可作为诊断标志物。
建议通过PubMed、Google Scholar等平台以“PIANP recombinant protein”、“PIANP function”等关键词检索真实文献。
**Background of PIANP Recombinant Protein**
PIANP (PSAP/Lactadherin and PLP family member), also known as C3orf36. is a relatively understudied protein implicated in various cellular processes, including immune regulation and neural development. Structurally, it belongs to the PSAP (prosaposin) and PLP (proteolipid protein) protein families, which are associated with lipid metabolism, membrane interactions, and cell signaling. PIANP is expressed in multiple tissues, with notable prominence in the brain, suggesting a potential role in neurological functions or disorders. Its exact molecular mechanisms, however, remain unclear, though studies link it to interactions with signaling pathways such as G protein-coupled receptors (GPCRs) or extracellular matrix components.
Recombinant PIANP protein is produced via biotechnological platforms (e.g., *E. coli* or mammalian expression systems) to enable functional and structural studies. The recombinant form allows researchers to explore PIANP's binding partners, signaling roles, and therapeutic potential in diseases like cancer, autoimmune disorders, or neurodegenerative conditions. For instance, its homology to lactadherin—a protein involved in phagocytosis and apoptosis—hints at possible immune-modulatory applications. Additionally, PIANP's neural expression pattern raises interest in its involvement in synaptic plasticity or neuroprotection.
Current research focuses on characterizing PIANP's biochemical properties, post-translational modifications, and tissue-specific isoforms. Challenges include elucidating its precise physiological ligands and downstream pathways. Recombinant PIANP serves as a critical tool for antibody development, *in vitro* assays, and preclinical models, bridging gaps in understanding its biological significance and translational relevance. Further studies may uncover its utility as a biomarker or therapeutic target in diverse pathologies.
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