关键信息
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基因名
SPACA3
- 应用
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别名
SPACA3；LYC3；LYZL3；SLLP1；Sperm acrosome membrane-associated Protein 3
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q8IXA5
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表达区间
1-215aa
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氨基酸序列
MVSALRGAPLIRVHSSPVSSPSVSGPRRLVSCLSSQSSALSQSGGGSTSAAGIEARSRALRRRWCPAGIMLLALVCLLSCLLPSSEAKLYGRCELARVLHDFGLDGYRGYSLADWVCLAYFTSGFNAAALDYEADGSTNNGIFQINSRRWCSNLTPNVPNVCRMYCSDLLNPNLKDTVICAMKITQEPQGLGYWEAWRHHCQGKDLTEWVDGCDF
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分子量
23.4 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
SPACA3 (Sperm Protein Associated with the Carboxyl Terminal of the Acrosome) is a protein that plays a significant role in sperm function and fertilization processes. Identified as a key player in sperm-egg interactions, SPACA3 is found in the acrosome of sperm cells, which is crucial for successful penetration of the oocyte during fertilization. Research has indicated that SPACA3 may contribute to the sperm’s ability to bind to the zona pellucida, the outer layer of the egg, thus facilitating fertilization. Its expression is primarily observed in testicular tissue and mature sperm, suggesting a specialized function in male fertility. Moreover, studies exploring SPACA3 have revealed its potential implications in male infertility, as variations in SPACA3 expression or mutations could hinder sperm functionality. In recent years, the production of recombinant SPACA3 has garnered interest, as it provides a platform for investigating the molecular mechanisms underlying sperm-egg recognition and binding. Understanding the structure and function of recombinant SPACA3 could pave the way for novel therapeutic strategies in addressing male infertility, as well as advancing assisted reproductive technologies. Overall, SPACA3 presents an intriguing subject for research due to its pivotal role in reproductive biology and its potential applications in clinical settings.
