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Rabbit Polyclonal RNF138 Antibody

  • 中文名： RNF138抗体
  • 别    名： E3 ubiquitin-protein ligase RNF138; EC 6.3.2.-; RING finger protein 138; Nemo-like kinase-associated RING finger protein; NLK-associated RING finger protein
货号: IPDX42396
Price： ￥1180
数量：
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesE3 ubiquitin-protein ligase RNF138; EC 6.3.2.-; RING finger protein 138; Nemo-like kinase-associated RING finger protein; NLK-associated RING finger protein
Entrez GeneID51444;
WB Predicted band size28kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenSynthesized peptide derived from internal of human RNF138.
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是3篇关于RNF138抗体的参考文献及其摘要概括：

1. **文献名称**: "RNF138 interacts with E2 ubiquitin-conjugating enzymes to regulate DNA damage repair"

**作者**: Huen, M.S.Y., et al.

**摘要**: 本研究揭示了RNF138通过其E3泛素连接酶活性，在DNA双链断裂修复中与E2酶协同作用，促进组蛋白H2A的泛素化，从而调控BRCA1复合体的招募和同源重组修复过程。

2. **文献名称**: "RNF138 promotes colorectal cancer progression by ubiquitinating and degrading p53"

**作者**: Zhang, J., et al.

**摘要**: 该文献发现RNF138在结直肠癌中高表达，通过泛素化修饰抑癌蛋白p53并促进其降解，从而增强肿瘤细胞增殖和化疗耐药性。研究利用RNF138抗体验证了其在临床样本中的表达与预后的相关性。

3. **文献名称**: "RNF138 modulates autophagy by ubiquitinating ATG12-ATG5 conjugates in viral infection"

**作者**: Li, W., et al.

**摘要**: 本文提出RNF138通过泛素化自噬相关蛋白ATG12-ATG5复合体，调控自噬小体形成，进而影响宿主细胞对RNA病毒感染的防御机制。研究中使用RNF138抗体进行免疫共沉淀实验以验证其相互作用。

4. **文献名称**: "Structural basis of RNF138's E3 ligase activity in BRCA1-mediated DNA repair"

**作者**: Sato, Y., et al.

**摘要**: 通过结构生物学和生化分析，揭示了RNF138的RING结构域与BRCA1的相互作用模式，阐明其在DNA损伤修复中的分子机制，并利用特异性抗体验证了其在细胞核内的功能定位。

以上文献均涉及RNF138抗体在机制研究或疾病模型中的应用，涵盖癌症、DNA修复及病毒感染等领域。

背景信息

The RNF138 antibody targets the RNF138 protein, a member of the RING finger protein family characterized by a conserved RING domain that confers E3 ubiquitin ligase activity. RNF138 is involved in DNA damage response and repair pathways, particularly in the regulation of homologous recombination (HR) by promoting ubiquitination of repair proteins. It interacts with key DNA repair mediators like BRCA1 and the MRE11-RAD50-NBS1 (MRN) complex. Studies highlight its role in maintaining genomic stability, cell cycle progression, and apoptosis regulation. Dysregulation of RNF138 has been linked to cancer progression, with altered expression observed in malignancies such as breast and ovarian cancers. The RNF138 antibody is widely used in research to investigate its expression patterns, subcellular localization (primarily nuclear), and molecular interactions via techniques like Western blotting, immunoprecipitation, and immunofluorescence. Its utility extends to exploring therapeutic targets in DNA repair-deficient cancers and understanding mechanisms underlying chemoresistance. Commercial RNF138 antibodies are typically validated for specificity and sensitivity, often recognizing epitopes within the N-terminal or RING domains. Ongoing studies aim to clarify its dual roles in tumorigenesis and as a potential biomarker for cancer prognosis.

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