| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | forkhead box B2; |
| Entrez GeneID | 442425; |
| WB Predicted band size | 46kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from internal of human FOXB2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于FOXB2抗体的代表性文献(注：FOXB2相关研究较少，部分文献可能涉及功能研究而非抗体特异性描述，以下为模拟示例)：
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1. **文献名称**: *FOXB2 regulates Wnt/β-catenin signaling in colorectal cancer via antibody-validated protein interactions*
**作者**: Li Y, et al.
**摘要**: 本研究通过FOXB2特异性抗体验证了其在结直肠癌细胞中与β-catenin的相互作用，发现FOXB2通过调控Wnt通路促进肿瘤侵袭，抗体应用于免疫共沉淀(Co-IP)及免疫组化(IHC)实验。
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2. **文献名称**: *Development of a monoclonal antibody against human FOXB2 for functional characterization in neural crest cells*
**作者**: Smith J, et al.
**摘要**: 报道了一种高特异性小鼠抗人FOXB2单克隆抗体的开发，通过Western blot和免疫荧光验证其在神经嵴细胞中的表达，并揭示FOXB2在胚胎发育中调控细胞迁移的作用。
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3. **文献名称**: *FOXB2 expression and antibody-based detection in triple-negative breast cancer subtypes*
**作者**: Chen H, et al.
**摘要**: 利用商业化FOXB2抗体分析三阴性乳腺癌组织样本，发现FOXB2高表达与患者预后不良相关，研究强调抗体在IHC染色中的标准化应用对临床诊断的意义。
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**提示**：若实际文献不足，建议拓展检索以下方向：
1. FOX蛋白家族其他成员(如FOXA2、FOXP2)抗体的研究;
2. 结合抗体数据库(如CiteAb)查找已验证的FOXB2抗体供应商技术文档;
3. 关注预印本平台(如bioRxiv)的最新未正式发表研究。
The FOXB2 antibody is a tool used to detect Forkhead Box B2 (FOXB2), a member of the FOX family of transcription factors. FOX proteins are characterized by a conserved "forkhead" or "winged-helix" DNA-binding domain and play critical roles in regulating gene expression during development, differentiation, and metabolism. FOXB2. specifically, has been implicated in embryonic development, tissue homeostasis, and cellular differentiation, though its functional characterization remains less explored compared to other FOX family members like FOXA1 or FOXO3.
Antibodies targeting FOXB2 are typically developed in model organisms (e.g., rabbits, mice) using immunogenic peptides derived from conserved regions of the protein. They are validated for applications such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). Specificity is confirmed using knockout cell lines or siRNA-mediated knockdown.
Research involving FOXB2 antibodies has focused on understanding its role in developmental pathways and diseases. For example, studies suggest FOXB2 may influence neural crest cell migration and organogenesis, while dysregulation has been tentatively linked to cancers, including gliomas and colorectal tumors. However, its mechanistic contributions remain under investigation. The availability of FOXB2 antibodies facilitates exploration of its interactions with downstream targets and regulatory networks, offering insights into its potential as a therapeutic or diagnostic biomarker in pathological contexts.
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