| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Gamma-aminobutyric acid receptor subunit alpha-4; GABA(A) receptor subunit alpha-4; |
| Entrez GeneID | 2557; |
| WB Predicted band size | 62kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from internal of human GABRA4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于GABRA4抗体的3篇参考文献示例(注：文献为虚拟示例，实际文献需通过学术数据库检索)：
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1. **文献名称**: *"GABRA4 subunit expression in autism spectrum disorder: A comparative immunohistochemical study"*
**作者**: Smith J, et al.
**摘要**: 本研究使用特异性GABRA4抗体，通过免疫组化分析自闭症患者与健康对照组脑组织样本。结果显示，自闭症患者前额叶皮层中GABRA4蛋白表达显著降低，提示其可能与GABA能信号异常相关。
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2. **文献名称**: *"Developmental changes in GABAA receptor α4 subunit localization in rodent brain"*
**作者**: Lee H, et al.
**摘要**: 通过Western blot和免疫荧光技术结合GABRA4抗体，研究者发现α4亚基在大鼠发育早期海马和丘脑中表达上调，成年后分布模式改变，表明该亚基在神经环路成熟中的作用。
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3. **文献名称**: *"Antibody validation for GABRA4 in human and mouse models of epilepsy"*
**作者**: Garcia R, et al.
**摘要**: 本文验证了多种商业化GABRA4抗体的特异性，并应用于癫痫模型脑组织检测。研究发现，在颞叶癫痫患者中，GABRA4蛋白水平异常升高，可能反映抑制性突触重塑代偿机制。
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如需具体文献，建议通过PubMed或Google Scholar检索关键词“GABRA4 antibody”“GABRA4 expression”或结合研究领域(如“epilepsy”“autism”)筛选。
The GABRA4 antibody is a research tool designed to specifically detect the GABRA4 protein, a subunit of the gamma-aminobutyric acid type A (GABAₐ) receptor. GABAₐ receptors are ligand-gated chloride channels that mediate fast inhibitory neurotransmission in the central nervous system. These receptors are pentameric complexes, typically composed of combinations of α, β, γ, δ, ε, or θ subunits. The GABRA4 gene encodes the α4 subunit, which is expressed predominantly in the thalamus, hippocampus, and cortex, and contributes to the receptor's pharmacological and functional diversity.
GABRA4-containing receptors are associated with neurodevelopmental and psychiatric disorders. Studies link GABRA4 polymorphisms or altered expression to conditions such as epilepsy, autism spectrum disorders, and anxiety. The α4 subunit is notable for its role in extrasynaptic receptors, which regulate tonic inhibition and are sensitive to neurosteroids and ethanol. Research using GABRA4 antibodies often focuses on mapping receptor distribution, quantifying expression changes in disease models, or exploring responses to therapeutic agents.
Validated GABRA4 antibodies are critical for techniques like immunohistochemistry, Western blotting, or immunofluorescence, enabling precise localization and analysis of subunit-specific receptor dynamics. Commercial antibodies are typically raised against peptide sequences unique to the α4 subunit, with validation in knockout models to ensure specificity. These tools advance understanding of GABAₐ receptor biology and their implications in neurological pathologies and drug development.
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