| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Activator-recruited cofactor 32 kDa component; ARC32; MED8; Mediator complex subunit 8; Mediator of RNA polymerase II transcription subunit 8 |
| Entrez GeneID | 112950; |
| WB Predicted band size | 29kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from Internal of human MED8. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于MED8抗体的参考文献示例(内容基于真实研究主题，但文献信息为模拟概括)：
1. **文献名称**: "The Mediator complex subunit MED8 interacts with E2F1 to regulate cell cycle progression in human cells"
**作者**: Zhang et al.
**摘要**: 本研究利用MED8特异性抗体通过免疫共沉淀(Co-IP)和染色质免疫沉淀(ChIP)技术，揭示了MED8与转录因子E2F1的相互作用，调控细胞周期相关基因的表达，影响癌细胞增殖。
2. **文献名称**: "Characterization of MED8 in Arabidopsis transcriptional regulation during stress response"
**作者**: Lee et al.
**摘要**: 通过Western blot和免疫荧光技术，作者使用植物来源的MED8抗体证明了MED8在中介体复合体中的结构作用，并发现其参与植物对干旱和盐胁迫的转录响应。
3. **文献名称**: "MED8 is essential for early embryonic development in mice"
**作者**: Smith et al.
**摘要**: 该研究利用MED8敲除小鼠模型及抗体标记技术，发现MED8缺失导致胚胎致死，并通过RNA-seq分析表明MED8通过调控多能性基因(如Oct4)维持胚胎干细胞自我更新。
4. **文献名称**: "Development and validation of a monoclonal antibody specific for human MED8"
**作者**: Tanaka et al.
**摘要**: 文章详细描述了针对人源MED8蛋白的单克隆抗体制备流程，验证了抗体在ELISA、免疫组化中的特异性，为后续MED8功能研究提供可靠工具。
---
注：以上文献信息为模拟概括，实际文献需通过学术数据库(如PubMed、Web of Science)检索确认。
The MED8 antibody targets the MED8 protein, a subunit of the Mediator complex—a multi-protein assembly essential for regulating RNA polymerase II-dependent transcription in eukaryotes. The Mediator complex acts as a molecular bridge, facilitating communication between transcription factors and the basal transcriptional machinery. MED8. along with other subunits, contributes to the structural integrity and functional specificity of the complex, playing roles in chromatin remodeling, transcription initiation, and post-initiation regulatory processes.
MED8 antibodies are widely used in research to investigate gene expression mechanisms, particularly in studies involving chromatin immunoprecipitation (ChIP), co-immunoprecipitation (Co-IP), and immunofluorescence. These tools help map MED8's genomic binding sites, assess its interactions with other Mediator subunits, and explore its localization within cellular compartments. Dysregulation of MED8 has been implicated in developmental disorders and cancers, making its study relevant for understanding disease pathways.
Commercial MED8 antibodies are typically raised in hosts like rabbits or mice, with validation across applications such as Western blotting and immunohistochemistry. Researchers often verify specificity using knockout cell lines or siRNA-mediated knockdown. Despite its conserved role, MED8's functional nuances may vary between species, necessitating antibody validation in relevant models. Ongoing studies aim to clarify MED8's tissue-specific roles and its potential as a therapeutic target in transcriptional dysregulation diseases.
×
