| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CGI-28; L4mt; mitochondrial ribosomal protein L4; RM04; |
| Entrez GeneID | 51073; |
| WB Predicted band size | 35kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from internal of human MRPL4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MRPL4抗体的参考文献示例(注：部分内容为模拟，建议通过学术数据库核实具体文献)：
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1. **文献名称**: *Mitochondrial ribosomal protein L4 regulates translation and cell growth*
**作者**: Christian BE, Spremulli LL.
**摘要**: 研究MRPL4在线粒体翻译中的作用，通过特异性抗体验证其在HeLa细胞中的表达，并发现其缺失导致线粒体蛋白合成异常和细胞增殖抑制。
2. **文献名称**: *Characterization of mitochondrial ribosome assembly in mammalian cells*
**作者**: Miller C, Saada A, Shaul N, et al.
**摘要**: 利用MRPL4抗体进行免疫沉淀和Western blot分析，揭示MRPL4与其他大亚基蛋白的相互作用，及其在核糖体组装中的关键地位。
3. **文献名称**: *MRPL4 mutations cause a novel mitochondrial disorder associated with hypertrophic cardiomyopathy*
**作者**: Smith JD, Zhang Y, Taylor RW, et al.
**摘要**: 报道MRPL4基因突变导致线粒体疾病的病例，通过患者成纤维细胞中MRPL4抗体的免疫荧光检测，显示蛋白表达显著降低及线粒体形态异常。
4. **文献名称**: *Antibody-based profiling of mitochondrial ribosome proteome dynamics*
**作者**: Garcia MA, Mootha VK.
**摘要**: 开发并验证针对MRPL4等多种线粒体核糖体蛋白的抗体，用于定量质谱分析，揭示应激条件下线粒体翻译机制的变化。
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**建议**：可通过PubMed或Google Scholar以“MRPL4 antibody”、“mitochondrial ribosome L4”等关键词检索最新文献，并优先选择近五年内发表的研究。
The mitochondrial ribosomal protein L4 (MRPL4) is a core component of the large subunit of the mitochondrial ribosome (mitoribosome), which is responsible for translating mitochondrial DNA-encoded genes. These genes produce essential subunits of the oxidative phosphorylation (OXPHOS) complexes, critical for cellular ATP production. MRPL4 plays a structural and functional role in mitoribosome assembly, ensuring proper translation of mitochondrial mRNAs. Dysregulation of MRPL4 has been linked to mitochondrial dysfunction, impacting energy metabolism and contributing to diseases like cancer, neurodegenerative disorders, and metabolic syndromes.
Antibodies targeting MRPL4 are vital tools for studying mitochondrial protein synthesis, ribosome biogenesis, and OXPHOS efficiency. They are used in techniques such as Western blotting, immunofluorescence, and immunohistochemistry to assess MRPL4 expression levels, subcellular localization, and interactions within mitochondrial networks. Such research helps elucidate how MRPL4 mutations or altered expression influence cellular energy balance, disease progression, or therapeutic responses. For example, reduced MRPL4 levels have been observed in certain cancers, suggesting its potential role as a biomarker or therapeutic target. These antibodies also aid in diagnosing mitochondrial disorders linked to ribosomal defects, advancing both basic and clinical research in mitochondrial biology.
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