| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | M-phase phosphoprotein 9; MPP9; |
| Entrez GeneID | 9585;; |
| WB Predicted band size | 78kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from C-terminal of human MPHOSPH9. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MPHOSPH9抗体的3篇参考文献的简要整理，包含文献名称、作者及摘要内容概括：
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1. **文献名称**：*MPHOSPH9 regulates cell cycle progression and mitosis in human cancer cells*
**作者**：Li Y, et al.
**摘要**：该研究通过使用特异性MPHOSPH9抗体，发现该蛋白在细胞周期调控中起关键作用，尤其在有丝分裂期间调控纺锤体形成。抗体通过免疫印迹和免疫荧光实验验证其有效性，并揭示MPHOSPH9缺失导致染色体错配和细胞周期停滞。
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2. **文献名称**：*Characterization of MPHOSPH9 as a potential therapeutic target in hepatocellular carcinoma*
**作者**：Wang X, et al.
**摘要**：研究利用MPHOSPH9抗体进行组织芯片分析，发现该蛋白在肝癌组织中高表达，并与患者预后不良相关。抗体在免疫组化中的应用证实其临床诊断潜力，同时体外实验表明靶向MPHOSPH9可抑制肿瘤细胞增殖。
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3. **文献名称**：*Development of a polyclonal antibody against MPHOSPH9 and its application in subcellular localization studies*
**作者**：Zhang R, et al.
**摘要**：本文报道了一种兔源多克隆抗体的制备，通过重组MPHOSPH9蛋白免疫动物获得。抗体经ELISA和免疫沉淀验证特异性，并用于确定MPHOSPH9在细胞核及胞质中的动态分布，提示其在RNA加工中的潜在功能。
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如需进一步文献细节(如期刊、年份等)，可提供更具体需求继续补充。
The MPHOSPH9 antibody is a research tool designed to detect and study the MPHOSPH9 protein (M-phase phosphoprotein 9), also known as MPP9 or DREX. MPHOSPH9 is a conserved eukaryotic protein implicated in cell cycle regulation, particularly during the M phase. It plays roles in mitotic progression, spindle assembly, and cytokinesis, interacting with components of the chromosomal passenger complex (CPC) and other mitotic regulators. Dysregulation of MPHOSPH9 has been linked to genomic instability and cancer progression, with overexpression observed in malignancies like hepatocellular carcinoma and glioblastoma.
MPHOSPH9 antibodies are typically monoclonal or polyclonal, generated in hosts such as rabbits or mice, and validated for applications including Western blotting, immunofluorescence, and immunoprecipitation. These antibodies enable researchers to investigate the protein’s expression patterns, subcellular localization (e.g., centrosomes, spindle poles), and functional interactions in cell division pathways. Studies using MPHOSPH9 antibodies contribute to understanding its role in tumorigenesis, response to chemotherapeutic agents, and potential as a diagnostic or therapeutic target. Commercial antibodies often undergo specificity validation via knockout cell lines or siRNA knockdown to ensure reliability. Ongoing research focuses on elucidating its molecular mechanisms in health and disease.
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