Rabbit Polyclonal MPRIP Antibody

The MPRIP (Myosin Phosphatase Rho Interacting Protein) antibody is a tool used to study the role of MPRIP, a multidomain scaffold protein involved in cytoskeletal dynamics and Rho signaling regulation. MPRIP interacts with myosin phosphatase (MP) and RhoA/ROCK pathways, modulating actomyosin contractility, cell adhesion, and migration. It contains functional domains, such as the Rho-binding domain (RBD) and coiled-coil regions, enabling interactions with critical regulators like RhoA, ROCK, and MP subunits. Dysregulation of MPRIP is implicated in pathological processes, including cancer metastasis, cardiovascular remodeling, and neurological disorders, due to its influence on cell motility, proliferation, and apoptosis. Researchers employ MPRIP antibodies in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess protein expression, localization, and post-translational modifications in tissues or cultured cells. These studies help clarify MPRIP’s role in maintaining cytoskeletal integrity, mediating mechanotransduction, and its crosstalk with signaling cascades like TGF-β or Wnt. The antibody’s specificity and validation (e.g., knockout validation) are critical for ensuring reliable data in exploring MPRIP as a potential therapeutic target or biomarker in diseases characterized by aberrant Rho signaling or cytoskeletal dysfunction.