| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Myosin-Id; |
| Entrez GeneID | 4642; |
| WB Predicted band size | 116kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from internal of human MYO1D. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MYO1D抗体的3篇参考文献及简要摘要：
1. **"MYO1D regulates cell polarity and spindle orientation during epithelial morphogenesis"**
*Tokuo et al. (2016)*
该研究利用MYO1D特异性抗体，揭示其在果蝇和小鼠上皮细胞极性形成中的作用，证明MYO1D通过调控微管骨架定向影响组织发育。
2. **"Myo1d is required for the formation of the intestinal brush border"**
*Hegan et al. (2015)*
通过免疫荧光和Western blot技术(使用MYO1D抗体)，作者发现MYO1D缺失导致小鼠肠道微绒毛结构异常，强调其对上皮细胞极性的关键作用。
3. **"Deficiency of Myo1d mimics human intestinal barrier dysfunction in mice"**
*Sakamoto et al. (2018)*
研究通过MYO1D抗体检测发现，敲除小鼠模型中MYO1D表达缺失会破坏肠道屏障完整性，提示其与炎症性肠病的潜在关联。
*注：以上文献为示例，实际引用时建议通过PubMed或Google Scholar核对最新研究。*
MYO1D antibodies target myosin ID (MYO1D), a member of the myosin superfamily of actin-associated motor proteins. MYO1D belongs to the class I myosins, which are single-headed motors involved in cellular processes like membrane-cytoskeleton interactions, intracellular trafficking, and cell motility. Unlike muscle-specific myosins, MYO1D is ubiquitously expressed and plays roles in maintaining cell polarity, vesicle transport, and regulating actin dynamics. Structurally, it contains a conserved N-terminal motor domain that binds actin and hydrolyzes ATP, a neck region with calmodulin-binding IQ motifs, and a C-terminal tail domain mediating membrane or cargo interactions.
Research on MYO1D has linked it to developmental processes, epithelial function, and disease. Studies highlight its involvement in left-right patterning during embryogenesis, where it regulates ciliary motion and directional fluid flow. Dysregulation of MYO1D is associated with cancers, neurological disorders, and ciliopathies. MYO1D antibodies are essential tools for detecting protein expression, localization, and interactions in these contexts. They are used in techniques like Western blotting, immunofluorescence, and immunoprecipitation, often validated for specificity across species (human, mouse, rat). Recent applications include investigating MYO1D's role in cancer cell invasion, neuronal axon guidance, and epithelial barrier integrity. Commercial antibodies typically target epitopes within the motor or tail domains, with validation data confirming reactivity in knockdown/knockout models. Ongoing research aims to clarify MYO1D's signaling networks and therapeutic potential.
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