Rabbit Polyclonal NDUFA3 Antibody

The NDUFA3 antibody is a research tool used to detect the NDUFA3 protein, a critical subunit of mitochondrial Complex I (NADH:ubiquinone oxidoreductase) in the electron transport chain. Complex I facilitates NADH oxidation and proton translocation, essential for ATP production. NDUFA3. encoded by the nuclear *NDUFA3* gene, is a 9 kDa hydrophobic protein located in the membrane arm of Complex I. It plays a structural and functional role in Complex I assembly and stability. Antibodies against NDUFA3 are typically developed in rabbits or mice using synthetic peptides or recombinant proteins as immunogens. They are widely employed in techniques like Western blotting, immunohistochemistry, and immunofluorescence to study NDUFA3 expression, localization, and involvement in mitochondrial disorders, such as Leigh syndrome, neurodegenerative diseases, or cancer. Reduced NDUFA3 levels have been linked to impaired oxidative phosphorylation and increased reactive oxygen species (ROS), contributing to cellular dysfunction. Researchers also use these antibodies to explore tissue-specific expression patterns and validate CRISPR/Cas9-mediated gene knockout models. Commercial NDUFA3 antibodies are often validated for specificity using knockdown/knockout controls or mass spectrometry. However, cross-reactivity with unrelated proteins may occur, necessitating careful optimization of experimental conditions.