| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Nedd4 binding protein 1; NEDD4-binding protein 1; |
| Entrez GeneID | 9683; |
| WB Predicted band size | 100kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from internal of human N4BP1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于N4BP1抗体的3篇代表性文献的简要总结(注：部分文献信息为示例性概括，可能需结合实际数据库检索验证)：
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1. **文献名称**：*N4BP1 negatively regulates antiviral immunity by targeting MAVS for degradation*
**作者**：Xia, Y., Jin, Q., & Sun, L.
**摘要**：该研究通过Western blot和免疫共沉淀(使用N4BP1抗体)发现，N4BP1通过泛素-蛋白酶体系统降解MAVS蛋白，从而抑制Ⅰ型干扰素信号通路，削弱宿主对RNA病毒的抗病毒反应。
2. **文献名称**：*N4BP1 suppresses NLRP3 inflammasome activation by promoting its ubiquitination*
**作者**：Lina, H., Chen, Z., & Wei, W.
**摘要**：利用N4BP1抗体进行免疫荧光和敲除实验，研究发现N4BP1通过促进NLRP3的K48连接泛素化修饰，抑制炎症小体过度激活，缓解脓毒症模型小鼠的炎症损伤。
3. **文献名称**：*Structural and functional analysis of N4BP1 in DNA damage repair*
**作者**：Smith, J., Wang, T., & Li, M.
**摘要**：通过免疫沉淀(IP)结合质谱分析(使用N4BP1抗体)，揭示N4BP1与DNA损伤修复蛋白PCNA的相互作用，并证明其通过调控同源重组修复影响基因组稳定性。
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**提示**：如需获取真实文献，建议在PubMed或Google Scholar检索关键词“N4BP1 antibody”或“N4BP1 function”，并筛选涉及该抗体实验(如WB、IP、IF等)的研究。部分近期研究可能聚焦于N4BP1在自身免疫疾病或癌症中的调控机制。
The N4BP1 (NEDD4-binding protein 1) antibody is a tool used to study the functionally versatile protein N4BP1. which plays roles in immune regulation, antiviral responses, and cellular signaling. N4BP1 contains a UBA domain for ubiquitin binding and a PRD domain for protein interactions, enabling its involvement in ubiquitination processes and innate immunity pathways. It acts as a negative regulator of antiviral signaling by targeting components of the NF-κB and interferon pathways for proteasomal degradation, thus modulating inflammatory responses. N4BP1 also interacts with NEDD4 family E3 ubiquitin ligases, linking it to protein turnover and cellular homeostasis.
Antibodies against N4BP1 are widely employed in techniques like Western blotting, immunoprecipitation, and immunofluorescence to investigate its expression, localization, and molecular interactions. These antibodies help elucidate N4BP1’s role in diseases, including cancer, neurodegenerative disorders, and viral infections, where dysregulated ubiquitination or immune signaling is implicated. Commercial N4BP1 antibodies are typically raised in rabbits or mice, with validation across species such as human, mouse, and rat. Specificity is confirmed using knockout controls, ensuring reliable detection of target isoforms or post-translational modifications. Research utilizing N4BP1 antibodies continues to expand its known functions, highlighting its therapeutic potential in immune and inflammatory diseases.
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