关键信息
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基因名
OTOF
- 应用
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别名
OTOF；FER1L2；Otoferlin
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9HC10
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表达区间
1461-1868aa
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氨基酸序列
KVPLPEDVSREAGYDSTYGMFQGIPSNDPINVLVRVYVVRATDLHPADINGKADPYIAIRLGKTDIRDKENYISKQLNPVFGKSFDIEASFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRFYSKHRATCGIAQTYSTHGYNIWRDPMKPSQILTRLCKDGKVDGPHFGPPGRVKVANRVFTGPSEIEDENGQRKPTDEHVALLALRHWEDIPRAGCRLVPEHVETRPLLNPDKPGIEQGRLELWVDMFPMDMPAPGTPLDISPRKPKKYELRVIIWNTDEVVLEDDDFFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYLFPFDYLAAEEKIVISKKESMFSWDETEYKIPARLTLQIWDADHFSADDFLGAIELDLNRFPRGAKTAKQCTMEM
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分子量
52.6 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
OTOF (Otoferlin) is a crucial protein that plays a significant role in auditory signaling and is primarily expressed in the inner hair cells of the cochlea, which are essential for sound transduction. Mutations in the OTOF gene are linked to auditory neuropathy and profound hearing loss, making OTOF a subject of intensive research to understand its function in auditory pathways and potential therapeutic avenues. The protein is involved in the regulation of synaptic vesicle fusion and neurotransmitter release at the ribbon synapses of inner hair cells, which is crucial for converting sound waves into electrical signals that the brain can interpret. Recombination and characterization of OTOF in laboratory settings not only aid in elucidating its biological functions but also facilitate the exploration of gene therapy and other innovative treatments for hearing impairments associated with OTOF dysregulation. As the understanding of OTOF's mechanistic role advances, it opens new doors for developing targeted interventions for hearing loss, thereby impacting millions affected by auditory disorders worldwide.
