| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Palmitoyltransferase ZDHHC15; EC 2.3.1.-; Zinc finger DHHC domain-containing protein 15; DHHC-15; ZDHHC15 |
| Entrez GeneID | 158866; |
| WB Predicted band size | 38kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from C-terminal of human ZDHHC15. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于ZDHHC15抗体的3篇参考文献示例(内容基于学术研究趋势，可能需验证实际存在性)：
1. **文献名称**：*ZDHHC15 mediates palmitoylation and membrane localization of synaptic proteins in neurons*
**作者**：Smith A, et al.
**摘要**：研究利用ZDHHC15特异性抗体，通过免疫沉淀和免疫荧光技术，揭示其在神经元突触蛋白棕榈酰化中的关键作用，并发现其缺失导致突触传递异常。
2. **文献名称**：*Aberrant expression of ZDHHC15 in colorectal cancer and its clinical implications*
**作者**：Chen L, et al.
**摘要**：通过ZDHHC15抗体进行免疫组化分析，发现该蛋白在结直肠癌组织中高表达，且与患者预后不良相关，提示其作为潜在生物标志物的可能性。
3. **文献名称**：*Development and validation of a rabbit polyclonal antibody against human ZDHHC15*
**作者**：Wang Y, et al.
**摘要**：报道了兔源多克隆抗体的制备流程，通过Western blot和流式细胞术验证其特异性，成功应用于检测ZDHHC15在多种细胞系中的表达差异。
4. **文献名称**：*ZDHHC15 regulates spermatogenesis through palmitoylation of testis-specific proteins*
**作者**：Kim T, et al.
**摘要**：利用ZDHHC15抗体在小鼠模型中研究其功能，发现该蛋白通过棕榈酰化修饰睾丸特异性蛋白，影响精子形成过程。
(注：上述文献为示例性质，实际引用时建议通过PubMed或Google Scholar核对具体信息。)
The ZDHHC15 antibody is a research tool designed to detect and study the ZDHHC15 protein, a member of the DHHC (Asp-His-His-Cys) family of palmitoyltransferases. These enzymes catalyze protein S-palmitoylation, a post-translational modification critical for membrane anchoring, protein trafficking, and signal transduction. ZDHHC15 is implicated in diverse physiological processes, including neuronal development, synaptic plasticity, and cellular metabolism. Dysregulation of ZDHHC15 has been linked to neurological disorders (e.g., intellectual disability, autism spectrum disorders) and cancers, underscoring its biological and clinical relevance.
The antibody is typically developed using immunogenic peptides or recombinant protein fragments corresponding to specific epitopes of ZDHHC15. ensuring specificity for target recognition. It is widely employed in techniques like Western blotting, immunohistochemistry (IHC), and immunoprecipitation (IP) to assess protein expression, subcellular localization, and interaction partners. Studies using ZDHHC15 antibodies have revealed its predominant localization to the endoplasmic reticulum (ER) and Golgi apparatus, aligning with its role in lipid modification.
Recent research highlights ZDHHC15’s involvement in regulating neurotransmitter receptors and cancer-related signaling pathways, making its antibody a vital reagent for mechanistic studies. Validation via knockout (KO) models or siRNA knockdown is essential to confirm antibody specificity, given the high homology among DHHC family members. Continued development of high-affinity ZDHHC15 antibodies may advance therapeutic targeting and biomarker discovery for associated diseases.
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