关键信息
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基因名
LATS2
- 应用
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别名
FLJ13161; Kinase phosphorylated during mitosis protein; KPM; Large tumor suppressor homolog 2; Large tumor suppressor homolog 2 Drosophila; Large tumor suppressor kinase 2; LATS large tumor suppressor Drosophila homolog 2; LATS large tumor suppressor homolog 2
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9NRM7
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表达区间
488-1088aa
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氨基酸序列
AAEGLDAKEEHALALGGAGAFPLDVEYGGPDRRCPPPPYPKHLLLRSKSE QYDLDSLCAGMEQSLRAGPNEPEGGDKSRKSAKGDKGGKDKKQIQTSPVP VRKNSRDEEKRESRIKSYSPYAFKFFMEQHVENVIKTYQQKVNRRLQLEQ EMAKAGLCEAEQEQMRKILYQKESNYNRLKRAKMDKSMFVKIKTLGIGAF GEVCLACKVDTHALYAMKTLRKKDVLNRNQVAHVKAERDILAEADNEWVV KLYYSFQDKDSLYFVMDYIPGGDMMSLLIRMEVFPEHLARFYIAELTLAI ESVHKMGFIHRDIKPDNILIDLDGHIKLTDFGLCTGFRWTHNSKYYQKGS HVRQDSMEPSDLWDDVSNCRCGDRLKTLEQRARKQHQRCLAHSLVGTPNY IAPEVLLRKGYTQLCDWWSVGVILFEMLVGQPPFLAPTPTETQLKVINWE NTLHIPAQVKLSPEARDLITKLCCSADHRLGRNGADDLKAHPFFSAIDFS SDIRKQPAPYVPTISHPMDTSNFDPVDEESPWNDASEGSTKAWDTLTSPN NKHPEHAFYEFTFRRFFDDNGYPFRCPKPSGAEASQAESSDLESSDLVDQ TEGCQPVYV
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分子量
110 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
LATS2, or Large Tumor Suppressor 2, is a crucial protein in the Hippo signaling pathway, which plays a significant role in regulating cell growth, proliferation, and apoptosis. The dysregulation of LATS2 has been implicated in various cancers, where its tumor-suppressive functions are diminished, allowing unchecked cellular expansion. Recent research indicates that LATS2 exerts its effects through controlling the activity of transcriptional co-activators such as YAP/TAZ, which, when overactivated, drive oncogenic pathways. Investigations into LATS2 have highlighted its potential as a therapeutic target, as restoring its function may reinstate normal cellular controls and inhibit tumor progression. Additionally, studies have explored the structural and functional properties of LATS2 through recombinant protein expression, aiming to elucidate its mechanisms and interactions at the molecular level. Researchers are employing various biochemical and biophysical techniques, such as X-ray crystallography and cryo-electron microscopy, to visualize the molecular architecture of LATS2 and its complexes. Understanding the precise role of LATS2 in the Hippo pathway and its interactions with other regulatory proteins could provide insights into novel strategies for cancer treatment, making it a promising focus of ongoing biomedical research.
