| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Myosin phosphatase-targeting subunit 3; MYPT3; |
| Entrez GeneID | 84988; |
| WB Predicted band size | 60kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | Synthesized peptide derived from internal of human PPP1R16A. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于PPP1R16A抗体的3篇参考文献及其摘要内容(文献为模拟示例，实际需根据具体研究补充)：
---
1. **文献名称**：*PPP1R16A regulates hepatitis B virus core protein nuclear localization through dephosphorylation*
**作者**：Choi Y, Kim JH, Park SJ
**摘要**：本研究通过免疫共沉淀和免疫荧光技术，利用抗PPP1R16A抗体揭示了其在HBV感染中通过调控核心蛋白磷酸化状态影响病毒颗粒组装，证实PPP1R16A与HBV核心蛋白的直接相互作用。
2. **文献名称**：*PPP1R16A promotes hepatocellular carcinoma progression by modulating cell cycle via PP1 phosphatase*
**作者**：Li X, Zhang W, Zhou H
**摘要**：通过Western blot和免疫组化分析，作者使用特异性PPP1R16A抗体发现其在肝癌组织中高表达，并通过激活PP1磷酸酶活性加速G1/S期转换，促进肿瘤增殖。
3. **文献名称**：*PPP1R16A interacts with AKT to regulate insulin signaling in hepatic stellate cells*
**作者**：Wang L, Liu T, Cheng M
**摘要**：该研究在肝纤维化模型中，利用PPP1R16A抗体进行Co-IP和流式细胞术，证明其通过AKT通路调控胰岛素信号传导，影响肝星状细胞活化和纤维化进程。
---
注：以上文献为示例，实际引用需查询PubMed、Web of Science等数据库获取具体研究。若需实验级抗体文献，可补充说明研究场景(如物种、应用方向)。
The PPP1R16A antibody targets a regulatory subunit of protein phosphatase 1 (PP1), a serine/threonine phosphatase involved in diverse cellular processes. PPP1R16A, also known as TIMAP or TGF-β1-inhibited membrane-associated protein, modulates PP1’s substrate specificity and localization. It contains nuclear localization signals (NLS), a PP1-binding motif, and a C-terminal RP repeats domain, enabling interactions with signaling molecules and cytoskeletal components.
Functionally, PPP1R16A is implicated in cell cycle regulation, apoptosis, and endothelial barrier integrity. It influences TGF-β signaling by counteracting Smad2/3 phosphorylation and interacts with Hippo pathway components (e.g., YAP1) to regulate cell proliferation. Its expression is tissue-specific, with high levels in vascular endothelial cells, liver, and kidneys.
Antibodies against PPP1R16A are used to study its expression, subcellular localization, and interactions in physiological or pathological contexts, such as cancer, fibrosis, or cardiovascular diseases. Western blotting, immunofluorescence, and immunohistochemistry are common applications. Dysregulation of PPP1R16A is linked to tumor progression, endothelial dysfunction, and metabolic disorders, making it a potential biomarker or therapeutic target. Commercial antibodies are typically validated for specificity using knockout controls or siRNA-based depletion.
×
