Cat: PA2000-524DB

Recombinant Human FHL1 Protein,His

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关键信息

  • 基因名

    FHL1

  • 应用

    SPRMSTBLIITCELISA细胞实验药物筛选

  • 别名

    FHL1;SLIM1;Four and a half LIM domains Protein 1

  • 种属

    Human

  • 表达系统

    E. coli

  • 标签

    His tag N-Terminus

  • 纯度

    Greater than 90% as determined by SDS-PAGE.

  • 蛋白编号

    Q13642

  • 表达区间

    1-280aa

  • 氨基酸序列

    MAEKFDCHYCRDPLQGKKYVQKDGHHCCLKCFDKFCANTCVECRKPIGAD SKEVHYKNRFWHDTCFRCAKCLHPLANETFVAKDNKILCNKCTTREDSPK CKGCFKAIVAGDQNVEYKGTVWHKDCFTCSNCKQVIGTGSFFPKGEDFYC VTCHETKFAKHCVKCNKAITSGGITYQDQPWHADCFVCVTCSKKLAGQRF TAVEDQYYCVDCYKNFVAKKCAGCKNPITGFGKGSSVVAYEGQSWHDYCF HCKKCSVNLANKRFVFHQEQVYCPDCAKKL

  • 分子量

    57 kDa

  • 内毒素

    < 1.0 EU per μg protein as determined by the LAL method.

  • 性状

    Freeze-dried powder

  • 缓冲液

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • 复溶方法

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • 个性化定制

    点位突变 标签定制 buffer定制 全长蛋白定制

  • 稳定性测试

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • 保存条件 & 期限

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • 运输条件

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

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背景信息

FHL1 (four and a half LIM domains protein 1) is a member of the LIM domain protein family, which is known for its role in various cellular processes, including muscle development, cell signaling, and gene transcription. Initial studies have shown that FHL1 is expressed in multiple tissues, particularly in skeletal muscle and cardiac tissue, suggesting its significant role in muscle-related functions. Research has indicated that FHL1 may be involved in regulating skeletal muscle hypertrophy and play a part in muscle degeneration diseases. Additionally, the dysregulation of FHL1 has been linked to several pathological conditions, including cardiomyopathy and muscular dystrophies. Efforts to produce recombinant FHL1 proteins have been undertaken to elucidate its molecular mechanisms and interactions at a biochemical level, as well as to explore its potential as a therapeutic target. The generation of these recombinant proteins allows researchers to investigate the structure-function relationships of FHL1, understand how it interacts with other proteins and cellular components, and assess its impact on cellular pathways. Furthermore, recombinant FHL1 can be utilized in high-throughput screening for small molecules or compounds that could modulate its function, opening avenues for the development of novel therapeutic strategies for muscle-related disorders. Given the complexities of muscle biology and the potential implications of FHL1 in muscular diseases, ongoing studies are critical to unravel its precise roles and therapeutic potential in both health and disease.

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