| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Zinc finger protein 446; Zinc finger protein with KRAB and SCAN domains 20; ZNF446; ZKSCAN20; |
| Entrez GeneID | 55663; |
| WB Predicted band size | 49kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from internal of human ZNF446. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于ZNF446抗体的假设性参考文献示例(请注意，这些为虚构内容，仅作格式参考)：
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1. **文献名称**：*ZNF446 Antibody Validation in Epigenetic Regulation Studies*
**作者**：Smith A, et al.
**摘要**：本研究验证了一种新型ZNF446抗体的特异性，并通过染色质免疫沉淀(ChIP)实验证明ZNF446与特定基因启动子区域的结合，揭示了其在DNA甲基化和肿瘤抑制基因沉默中的关键作用。
2. **文献名称**：*Role of ZNF446 in Stem Cell Differentiation Using Immunohistochemical Analysis*
**作者**：Zhang L, et al.
**摘要**：通过Western blot和免疫组化技术，作者发现ZNF446在胚胎干细胞分化过程中表达上调，其抗体标记显示该蛋白通过调控多能性转录因子(如OCT4)维持干细胞稳态。
3. **文献名称**：*ZNF446 Overexpression in Gastric Cancer: A Prognostic Marker Study*
**作者**：Lee J, et al.
**摘要**：利用ZNF446抗体对胃癌组织进行免疫组化分析，发现高表达ZNF446与患者预后不良显著相关，体外实验进一步表明其通过激活Wnt/β-catenin通路促进癌细胞增殖。
4. **文献名称**：*Development of a Polyclonal ZNF446 Antibody for Functional Genomics*
**作者**：Chen R, et al.
**摘要**：本文报道了一种兔源多克隆ZNF446抗体的制备及验证，该抗体成功应用于免疫共沉淀(Co-IP)和亚细胞定位研究，证实ZNF446主要定位于细胞核并参与转录抑制复合物。
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以上示例展示了不同研究场景中ZNF446抗体的应用，涵盖抗体开发、表观遗传机制、疾病关联等领域。实际文献需通过学术数据库(如PubMed)检索。
**Background of ZNF446 Antibody**
The ZNF446 antibody is a tool used to detect and study the zinc finger protein 446 (ZNF446), a member of the Krüppel-associated box (KRAB) domain-containing zinc finger protein family. ZNF446 is a transcription factor characterized by multiple C2H2-type zinc finger motifs, which enable sequence-specific DNA binding, and a KRAB domain at its N-terminus that mediates protein-protein interactions, often recruiting transcriptional corepressors. This protein is implicated in regulating gene expression, particularly in developmental processes and cellular differentiation. Studies suggest its involvement in modulating TGF-β and Wnt signaling pathways, potentially influencing cell proliferation, apoptosis, and carcinogenesis.
Antibodies targeting ZNF446 are widely used in research to investigate its expression patterns, subcellular localization (primarily nuclear), and functional roles. They are employed in techniques such as Western blotting (WB), immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). Commercial ZNF446 antibodies are typically raised in rabbits or mice, with validation in specific applications and species. Epitope specificity varies, with some antibodies recognizing the KRAB domain or specific zinc finger regions.
Research using ZNF446 antibodies has explored its dual role as a tumor suppressor or oncogene in different cancers, such as hepatocellular carcinoma and gastric cancer, as well as its potential association with neurodevelopmental disorders. These studies highlight its importance in both basic transcriptional regulation research and pathological analysis.
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