关键信息
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基因名
HCy
- 应用
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别名
HCy；S-methylmethionine--homocysteine S-methyltransferase BHMT2
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q12525
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表达区间
1-324aa
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氨基酸序列
MKRIPIKELIVEHPGKVLILDGGQGTELENRGININSPVWSAAPFTSESFWEPSSQERKVVEEMYRDFMIAGANILMTITYQANFQSISENTSIKTLAAYKRFLDKIVSFTREFIGEERYLIGSIGPWAAHVSCEYTGDYGPHPENIDYYGFFKPQLENFNQNRDIDLIGFETIPNFHELKAILSWDEDIISKPFYIGLSVDDNSLLRDGTTLEEISVHIKGLGNKINKNLLLMGVNCVSFNQSALILKMLHEHLPGMPLLVYPNSGEIYNPKEKTWHRPTNKLDDWETTVKKFVDNGARIIGGCCRTSPKDIAEIASAVDKYS
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分子量
40.7 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
Homocysteine (HCy) is a sulfur-containing amino acid that plays a significant role in various biological processes, including protein synthesis and methylation reactions. Elevated levels of homocysteine in the bloodstream, known as hyperhomocysteinemia, have been associated with a range of health issues, particularly cardiovascular diseases, neurodegenerative disorders, and complications during pregnancy. The metabolism of homocysteine involves several key enzymes and cofactors, including vitamins B6, B12, and folate, which work together to convert homocysteine into cysteine or methionine. Research into HCy recombinant proteins seeks to better understand HCy metabolism and its implications for human health. Recombinant technology allows for the production of homocysteine-related proteins, enabling scientists to study their structure and function in detail, as well as to explore potential therapeutic applications for conditions linked to abnormal HCy levels. The exploration of HCy's role in epigenetic regulation, inflammation, and cellular signaling further underscores the importance of understanding its biochemical pathways. By creating recombinant forms of enzymes involved in HCy metabolism, studies can elucidate their mechanisms of action and their influence on health, paving the way for new diagnostic and treatment strategies for diseases associated with HCy dysregulation.
