关键信息
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基因名
ATP5b
- 应用
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别名
ATP5b；ATP5B；ATPMB；ATPSB；ATP synthase subunit beta. mitochondrial
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P06576
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表达区间
48-529aa
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氨基酸序列
AQTSPSPKAGAATGRIVAVIGAVVDVQFDEGLPPILNALEVQGRETRLVLEVAQHLGESTVRTIAMDGTEGLVRGQKVLDSGAPIKIPVGPETLGRIMNVIGEPIDERGPIKTKQFAPIHAEAPEFMEMSVEQEILVTGIKVVDLLAPYAKGGKIGLFGGAGVGKTVLIMELINNVAKAHGGYSVFAGVGERTREGNDLYHEMIESGVINLKDATSKVALVYGQMNEPPGARARVALTGLTVAEYFRDQEGQDVLLFIDNIFRFTQAGSEVSALLGRIPSAVGYQPTLATDMGTMQERITTTKKGSITSVQAIYVPADDLTDPAPATTFAHLDATTVLSRAIAELGIYPAVDPLDSTSRIMDPNIVGSEHYDVARGVQKILQDYKSLQDIIAILGMDELSEEDKLTVSRARKIQRFLSQPFQVAEVFTGHMGKLVPLKETIKGFQQILAGEYDHLPEQAFYMVGPIEEAVAKADKLAEEHSS
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分子量
53.8 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
ATP5b, a crucial component of the ATP synthase complex, plays a significant role in mitochondrial energy production through oxidative phosphorylation. The ATP5b gene encodes the beta subunit of the ATP synthase F1 sector, which is essential for converting ADP and inorganic phosphate into ATP, the primary energy currency of the cell. Mutations in ATP5b have been associated with various mitochondrial disorders, leading to impaired ATP synthesis and a range of metabolic dysfunctions. Research into recombinant ATP5b proteins is vital for understanding the mechanisms of ATP synthase function and regulation, exploring its structural properties, and investigating how specific mutations affect its activity. By producing recombinant ATP5b, scientists can study its interactions within the ATP synthase complex and its role in maintaining mitochondrial health. Additionally, the recombinant protein serves as a valuable tool for drug screening and potential therapeutic applications aimed at ameliorating mitochondrial diseases. The study of ATP5b is not only significant for basic mitochondrial biology but also for the development of targeted interventions in a field increasingly recognized for its relevance to aging and metabolic disorders.
