| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下为模拟生成的关于ECFP Mouse抗体的参考文献示例(非真实文献，仅供格式参考)：
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1. **"Development and Characterization of a Monoclonal Mouse Antibody Against ECFP for Live-Cell Imaging"**
*Authors: Zhang L, et al.*
**摘要**: 本研究报道了一种新型小鼠单克隆抗体的开发，该抗体特异性识别增强型青色荧光蛋白(ECFP)，适用于活细胞成像和蛋白质定位分析。抗体经ELISA和免疫荧光验证，显示高亲和力与低交叉反应性。
2. **"Comparative Analysis of ECFP-Tag Recognition by Mouse-Derived Antibodies in Western Blotting"**
*Authors: Müller S, et al.*
**摘要**: 通过系统比较多种商品化小鼠抗ECFP抗体在Western blot中的表现，本文筛选出灵敏度最高(检测限达0.1 ng)的抗体，并验证其在重组蛋白检测中的应用潜力。
3. **"A Mouse Monoclonal Antibody Enables Rapid Purification of ECFP-Fusion Proteins via Immunoprecipitation"**
*Authors: Tanaka K, et al.*
**摘要**: 描述了一种高效小鼠单抗的开发，该抗体通过结合ECFP标签实现融合蛋白的一步免疫沉淀纯化，显著提高蛋白纯化效率，适用于高通量蛋白质组学研究。
4. **"In Vivo Application of ECFP-Specific Mouse Antibodies for Tumor Targeting in Murine Models"**
*Authors: Chen R, et al.*
**摘要**: 利用抗ECFP小鼠抗体偶联近红外探针，成功实现小鼠模型中ECFP标记肿瘤的体内靶向成像，为荧光标记技术的活体应用提供了新工具。
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注：以上内容为示例性虚构文献，实际文献需通过学术数据库(如PubMed、Web of Science)检索关键词“ECFP antibody mouse”获取。
ECFP (Extracellular Filamentous Protein) mouse antibodies are immunotools developed to target specific epitopes on extracellular filamentous proteins, which play critical roles in cellular adhesion, structural support, and signal transduction. These antibodies are typically generated through hybridoma technology, where mice are immunized with purified or recombinant ECFP antigens to elicit a robust immune response. The resulting monoclonal antibodies exhibit high specificity and affinity, making them valuable in detecting ECFP expression in various experimental settings, including immunohistochemistry (IHC), immunofluorescence (IF), and Western blotting.
ECFP mouse antibodies are widely utilized in studying cell-matrix interactions, tissue architecture, and pathological processes like fibrosis or cancer metastasis. Their applications extend to biomedical research, where they help visualize extracellular matrix (ECM) dynamics or validate protein localization in disease models. Due to the conserved nature of many filamentous proteins across species, these antibodies often demonstrate cross-reactivity with homologs in humans, rats, and other mammals, enhancing their utility in translational studies.
Recent advancements in recombinant antibody engineering have further improved their performance, reducing batch-to-batch variability and enabling conjugation with fluorescent dyes or enzymes for multiplex assays. Despite their specificity, researchers must validate ECFP mouse antibodies in context-dependent assays to minimize off-target effects. Overall, they remain indispensable tools for dissecting extracellular protein functions in both physiological and pathological contexts.
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