| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/40000 | Human,Mouse,Rat |
| Aliases | CDC42EP5; BORG3; CEP5; Cdc42 effector protein 5; Binder of Rho GTPases 3 |
| Entrez GeneID | 148170; |
| WB Predicted band size | 22kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from the N-terminal region of human Cdc42EP5. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Cdc42EP5抗体的3篇参考文献的示例(注：以下内容为虚构示例，实际文献需根据具体研究检索)：
1. **文献名称**：*Cdc42EP5 regulates cell polarity and tight junction formation in epithelial cells*
**作者**：Smith A, et al.
**摘要**：该研究利用Cdc42EP5特异性抗体，通过免疫荧光和Western blot技术，揭示了Cdc42EP5在调控上皮细胞极性和紧密连接形成中的作用，证明其通过与Cdc42相互作用影响细胞骨架重组。
2. **文献名称**：*CDC42EP5 expression correlates with metastatic potential in colorectal cancer*
**作者**：Li X, et al.
**摘要**：研究通过Cdc42EP5抗体检测结直肠癌组织样本，发现其高表达与肿瘤转移和不良预后显著相关，并验证了该蛋白通过调控侵袭伪足促进癌细胞迁移的机制。
3. **文献名称**：*Cdc42EP5 modulates exocytosis via interaction with septin complexes*
**作者**：Garcia-Rivera D, et al.
**摘要**：本文采用Cdc42EP5抗体进行免疫共沉淀实验，发现Cdc42EP5通过与septin蛋白复合物结合参与囊泡运输和胞吐过程，揭示了其在分泌通路中的新功能。
(实际文献需通过PubMed、Google Scholar等平台以“Cdc42EP5 antibody”或“CDC42EP5 function”为关键词检索获取。)
The Cdc42EP5 antibody is a research tool designed to detect Cdc42 Effector Protein 5 (Cdc42EP5), a member of the CDC42 effector protein family. Cdc42EP5. also known as BORG2. interacts with the small GTPase CDC42. a critical regulator of cytoskeletal dynamics, cell polarity, and membrane trafficking. This protein is implicated in organizing the actin cytoskeleton by coordinating with other effector proteins, thereby influencing cell morphology, adhesion, and migration. Cdc42EP5 is particularly noted for its role in septin network assembly, which contributes to cellular compartmentalization and structural integrity.
Antibodies targeting Cdc42EP5 are commonly used in immunoblotting (Western blot), immunofluorescence, and immunoprecipitation to study its expression, localization, and interactions in various cell types. These antibodies help elucidate Cdc42EP5's involvement in physiological processes, such as tissue development and immune cell function, as well as pathological conditions, including cancer metastasis and inflammatory diseases. Dysregulation of Cdc42EP5 has been linked to altered cell motility and invasive phenotypes in malignancies, making it a potential biomarker or therapeutic target.
Researchers typically validate these antibodies using knockout cell lines or siRNA-mediated knockdown to ensure specificity. Commercial antibodies are often raised in rabbits or mice, with available monoclonal or polyclonal formats. Their applications span basic cell biology, cancer research, and studies of cellular mechanotransduction, underscoring their utility in exploring CDC42-mediated signaling pathways.
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