关键信息
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基因名
vsdF
- 应用
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别名
vsdF；Virulence Protein VsdF
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P24421
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表达区间
1-119aa
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氨基酸序列
MLRATKVCIYPTPEQAEHLNAQFGAVRFVYSKSLHIKKHAYQRHGVSLTPRKDIKPLLAVAKKFRKFRKFRKYAWLKEYDSIALQQAVINLDVAFSNCFNPKLKARFPMFKRKHGKLLG
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分子量
26.9 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
The study of VSDf recombinant proteins has garnered significant interest in the fields of molecular biology and biotechnology due to their crucial role in understanding the function of voltage-sensing domains (VSDs) in membrane proteins. VSDs are integral components of various ion channels, such as voltage-gated sodium, potassium, and calcium channels, which are essential for cellular excitability and signal transduction. Research into VSDf proteins focuses on utilizing recombinant DNA technology to produce these proteins in a controlled laboratory setting, enabling scientists to investigate their structure, function, and interaction with other cellular components. By analyzing VSDf, researchers aim to elucidate the mechanisms of voltage sensing and gating in ion channels, contributing to a deeper understanding of neuronal signaling and the physiological processes that are affected by ionic currents. Furthermore, insights gained from VSDf research have potential implications for the development of targeted therapies for voltage-related diseases, such as epilepsy and cardiac arrhythmias. Through the production of recombinant VSDf proteins, scientists can conduct biophysical and biochemical studies, leading to advancements in our knowledge of cellular communication and paving the way for innovative medical applications.
