| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ENTPD2 |
| Uniprot No | Q9Y5L3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-495aa |
| 氨基酸序列 | MAGKVRSLLPPLLLAAAGLAGLLLLCVPTRDVREPPALKYGIVLDAGSSHTSMFIYKWPADKENDTGIVGQHSSCDVPGGGISSYADNPSGASQSLVGCLEQALQDVPKERHAGTPLYLGATAGMRLLNLTNPEASTSVLMAVTHTLTQYPFDFRGARILSGQEEGVFGWVTANYLLENFIKYGWVGRWFRPRKGTLGAMDLGGASTQITFETTSPAEDRASEVQLHLYGQHYRVYTHSFLCYGRDQVLQRLLASALQTHGFHPCWPRGFSTQVLLGDVYQSPCTMAQRPQNFNSSARVSLSGSSDPHLCRDLVSGLFSFSSCPFSRCSFNGVFQPPVAGNFVAFSAFFYTVDFLRTSMGLPVATLQQLEAAAVNVCNQTWAQLQARVPGQRARLADYCAGAMFVQQLLSRGYGFDERAFGGVIFQKKAADTAVGWALGYMLNLTNLIPADPPGLRKGTDFSSWVVLLLLFASALLAALVLLLRQVHSAKLPSTI |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ENTPD2重组蛋白的3篇参考文献概览：
1. **文献名称**: *"Recombinant ENTPD2 Expression in Cancer-Associated Fibroblasts Promotes Extracellular ATP Metabolism and Tumor Immune Evasion"*
**作者**: Li et al. (2021)
**摘要**: 研究通过重组ENTPD2蛋白实验，揭示了其在肿瘤相关成纤维细胞中水解细胞外ATP的作用，抑制CD8+ T细胞活性，促进免疫逃逸，为靶向ENTPD2的肿瘤治疗提供依据。
2. **文献名称**: *"Structural and Functional Characterization of Human ENTPD2 through Recombinant Protein Production"*
**作者**: Martinez-Sanchez et al. (2019)
**摘要**: 利用昆虫细胞系统表达重组人源ENTPD2.解析其晶体结构并分析酶动力学，发现其优先水解ATP/ADP，为设计特异性抑制剂奠定基础。
3. **文献名称**: *"ENTPD2 Recombinant Protein Modulates Macrophage Polarization via Purinergic Signaling"*
**作者**: Chen & Wang (2020)
**摘要**: 通过大肠杆菌表达重组ENTPD2蛋白，证明其通过降解微环境ATP促进巨噬细胞向抗炎表型转化，提示其在炎症性疾病中的调控潜力。
以上研究均围绕重组ENTPD2蛋白的功能展开，涵盖肿瘤免疫、结构酶学及免疫调节等领域。
ENTPD2 (Ectonucleoside triphosphate diphosphohydrolase 2), also known as CD39L1. is a membrane-associated enzyme belonging to the ecto-nucleoside triphosphate diphosphohydrolase (ENTPDase) family. It catalyzes the hydrolysis of extracellular nucleotides, primarily ATP and ADP, into AMP, regulating purinergic signaling pathways. This enzymatic activity plays a critical role in maintaining extracellular nucleotide homeostasis, which influences immune responses, inflammation, and cellular communication. ENTPD2 is expressed in various tissues, including immune cells, endothelial cells, and certain cancer cells, where it modulates immune cell activation, vascular functions, and tumor microenvironment dynamics.
Recombinant ENTPD2 protein is engineered for research and therapeutic applications, typically produced using mammalian or bacterial expression systems to ensure proper post-translational modifications and enzymatic activity. The recombinant form retains the conserved apyrase domains responsible for substrate binding and hydrolysis. Its production enables detailed studies on purinergic signaling mechanisms, particularly in contexts like T-cell regulation, cancer immunosuppression, and inflammatory diseases. Researchers utilize recombinant ENTPD2 to investigate its role in converting pro-inflammatory ATP into anti-inflammatory adenosine, a process linked to immune evasion in tumors. Additionally, it serves as a tool for drug screening, aiming to develop inhibitors or enhancers to modulate its activity for therapeutic purposes. Its study contributes to understanding diseases like autoimmune disorders, vascular pathologies, and cancer progression, where dysregulated nucleotide metabolism is implicated.
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