关键信息
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基因名
PCMTD1
- 应用
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别名
PCMTD1;Protein-L-isoaspartate O-methyltransferase domain-containing Protein 1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q96MG8
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表达区间
1-220aa
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氨基酸序列
MGSSHHHHHH SSGLVPRGSH MGSMGGAVSAGEDNDDLIDNLKEAQYIRTERVEQAFRAIDRGDYYLEGYR DNAYKDLAWKHGNIHLSAPCIYSEVMEALKLQPGLSFLNLGSGTGYLSTM VGLILGPFGINHGIELHSDVVEYAKEKLESFIKNSDSFDKFEFCEPAFVV GNCLQIASDSHQYDRIYCGAGVQKDHENYMKILLKVGGILVMPIEDQLTQ IMRTGQNTWESKNILAVSFAPLV
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分子量
27 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
PCMTD1 (Protein-L-isoaspartate O-methyltransferase domain-containing protein 1) is a member of the methyltransferase family, implicated in the post-translational modification of proteins. Its primary role is to repair damaged proteins by methylating L-isoaspartate residues, which accumulate due to non-enzymatic reactions that occur over time, particularly under stress conditions. The presence of these non-canonical amino acids can adversely affect protein function, stability, and cellular homeostasis, leading to various diseases, including neurodegenerative disorders and age-related pathologies. Recent studies have highlighted the important function of PCMTD1 in cellular protection mechanisms, demonstrating its potential as both a biomarker for stress-related conditions and a therapeutic target. The exploration of PCMTD1 involves understanding its enzymatic activity, structural features, and regulatory pathways. Additionally, ongoing research aims to delineate its interactions with other proteins and cellular components, which can uncover novel insights into its role in maintaining protein integrity and cellular health. Understanding the molecular mechanisms and biological significance of PCMTD1 is crucial for developing strategies to mitigate protein misfolding and aggregation, thus advancing therapeutic approaches in age-associated diseases and neurodegeneration.
