关键信息
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基因名
APPBP1
- 应用
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别名
APPBP1；APPBP1；NEDD8-activating enzyme E1 regulatory subunit
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q13564
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表达区间
1-534aa
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氨基酸序列
MGSSHHHHHHSSGLVPRGSHMGSMAQLGKLLKEQKYDRQLRLWGDHGQEA LESAHVCLINATATGTEILKNLVLPGIGSFTIIDGNQVSGEDAGNNFFLQ RSSIGKNRAEAAMEFLQELNSDVSGSFVEESPENLLDNDPSFFCRFTVVV ATQLPESTSLRLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIESHPD NALEDLRLDKPFPELREHFQSYDLDHMEKKDHSHTPWIVIIAKYLAQWYS ETNGRIPKTYKEKEDFRDLIRQGILKNENGAPEDEENFEEAIKNVNTALN TTQIPSSIEDIFNDDRCINITKQTPSFWILARALKEFVAKEGQGNLPVRG TIPDMIADSGKYIKLQNVYREKAKKDAAAVGNHVAKLLQSIGQAPESISE KELKLLCSNSAFLRVVRCRSLAEEYGLDTINKDEIISSMDNPDNEIVLYL MLRAVDRFHKQQGRYPGVSNYQVEEDIGKLKSCLTGFLQEYGLSVMVKDD YVHEFCRYGAAEPHTIAAFLGGAAAQEVIKIITKQFVIFNNTYIYSGMSQ TSATFQL
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分子量
63 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
APPBP1, or Amyloid-beta Precursor Protein-Binding Protein 1, plays a critical role in cellular processes, particularly in relation to Alzheimer's disease and protein degradation pathways. This protein is involved in the regulation of the ubiquitin-proteasome system, which is essential for maintaining cellular homeostasis by degrading misfolded or damaged proteins. Dysregulation of APPBP1 has been implicated in the accumulation of amyloid-beta peptides, a hallmark of Alzheimer’s pathology. Recent studies have highlighted the significance of APPBP1 in mediating the interaction between the amyloid precursor protein and various signaling molecules, suggesting that it may influence the trajectory of neurodegenerative diseases. Furthermore, the recombinant expression of APPBP1 provides a valuable tool for studying its structural and functional properties, as well as its interactions with other proteins. Understanding the role of APPBP1 at the molecular level can aid in the development of novel therapeutic strategies aimed at modulating its activity and potentially mitigating the effects of Alzheimer's disease. Research on APPBP1 has expanded to investigate its involvement in cancer and other neurodegenerative disorders, marking it as a promising target for drug discovery and therapeutic intervention. The generation of recombinant APPBP1 proteins allows for in-depth biochemical assays, structural studies, and potential high-throughput screening approaches to identify small molecules that can modulate its activity, offering new avenues for disease-modifying therapies.
