| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | CRT |
| Uniprot No | P27797 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 18-417aa |
| 氨基酸序列 | EPAVYFKEQFLDGDGWTSRWIESKHKSDFGKFVLSSGKFYGDEEKDKGLQTSQDARFYALSASFEPFSNKGQTLVVQFTVKHEQNIDCGGGYVKLFPNSLDQTDMHGDSEYNIMFGPDICGPGTKKVHVIFNYKGKNVLINKDIRCKDDEFTHLYTLIVRPDNTYEVKIDNSQVESGSLEDDWDFLPPKKIKDPDASKPEDWDERAKIDDPTDSKPEDWDKPEHIPDPDAKKPEDWDEEMDGEWEPPVIQNPEYKGEWKPRQIDNPDYKGTWIHPEIDNPEYSPDPSIYAYDNFGVLGLDLWQVKSGTIFDNFLITNDEAYAEEFGNETWGVTKAAEKQMKDKQDEEQRLKEEEEDKKRKEEEEAEDKEDDEDKDEDEEDEEDKEEDEEEDVPGQAKDEL |
| 预测分子量 | 50.6kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于CRT(钙网蛋白)重组蛋白的文献概述：
---
1. **文献名称**：*Calreticulin exposure in immunogenic cell death: From signaling mechanisms to therapeutic opportunities*
**作者**：Krysko DV, et al.
**摘要**：该综述探讨了重组钙网蛋白(CRT)在免疫原性细胞死亡(ICD)中的关键作用，阐明了其在细胞表面暴露后激活树突状细胞及抗肿瘤免疫应答的分子机制，并讨论了其在癌症免疫治疗中的潜在应用。
---
2. **文献名称**：*Expression and purification of recombinant human calreticulin in Escherichia coli*
**作者**：Wang WA, et al.
**摘要**：研究描述了一种高效表达和纯化重组人源CRT的方法，通过优化大肠杆菌表达系统，获得高纯度、具有生物活性的CRT蛋白，为后续功能研究及药物开发提供基础。
---
3. **文献名称**：*Recombinant calreticulin modulates integrin-mediated adhesion via direct interaction with α-subunits*
**作者**：Coppolino MG, et al.
**摘要**：通过体外实验验证重组CRT与整合素α亚基的直接结合，揭示其调控细胞黏附、迁移及信号传导的分子机制，为CRT在炎症和癌症转移中的作用提供新见解。
---
4. **文献名称**：*Structural analysis of calreticulin: Insights into its chaperone function and interactions*
**作者**：Ellgaard L, et al.
**摘要**：利用X射线晶体学解析重组CRT的三维结构，阐明其作为分子伴侣的功能域及与钙离子结合的位点，揭示了CRT在蛋白质折叠和内质网质量控制中的关键作用。
---
以上文献涵盖CRT重组蛋白的功能机制、生产方法及结构解析，适用于基础研究与临床应用参考。
Calreticulin (CRT), a multifunctional endoplasmic reticulum (ER)-resident protein, plays critical roles in calcium homeostasis, protein folding quality control, and immune regulation. Initially identified as an ER chaperone, CRT assists in the proper folding of glycoproteins via the calnexin/calreticulin cycle and regulates intracellular calcium storage. Beyond its ER functions, CRT translocates to the cell surface under stress conditions, such as apoptosis or immunogenic cell death, where it acts as an "eat-me" signal to promote phagocytic clearance of dying cells and stimulate adaptive immunity.
Recombinant CRT (rCRT) is produced using genetic engineering techniques, typically through expression in bacterial (e.g., *E. coli*) or eukaryotic systems (e.g., mammalian or insect cells). Bacterial systems offer cost-effective production but lack post-translational modifications, while eukaryotic systems ensure proper folding and glycosylation. rCRT retains the functional domains of native CRT, including the N-terminal lectin domain, central proline-rich region, and C-terminal calcium-binding domain.
The therapeutic and research applications of rCRT are expanding. It serves as a tool to study CRT-mediated immune responses, particularly in cancer immunotherapy, where surface CRT exposure enhances tumor immunogenicity. rCRT also shows potential in vaccine development, acting as an adjuvant to improve antigen presentation. Additionally, it aids in investigating autoimmune diseases, as CRT autoantibodies are linked to conditions like lupus and rheumatoid arthritis. Challenges remain in optimizing production yields and maintaining structural stability, but rCRT continues to emerge as a versatile molecule bridging cellular stress responses and immune modulation.
×
