| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SPINT1 |
| Uniprot No | O43278 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 36-529aa |
| 氨基酸序列 | GPPPA PPGLPAGADC LNSFTAGVPG FVLDTNASVS NGATFLESPT VRRGWDCVRA CCTTQNCNLA LVELQPDRGE DAIAACFLIN CLYEQNFVCK FAPREGFINY LTREVYRSYR QLRTQGFGGS GIPKAWAGID LKVQPQEPLV LKDVENTDWR LLRGDTDVRV ERKDPNQVEL WGLKEGTYLF QLTVTSSDHP EDTANVTVTV LSTKQTEDYC LASNKVGRCR GSFPRWYYDP TEQICKSFVY GGCLGNKNNY LREEECILAC RGVQGGPLRG SSGAQATFPQ GPSMERRHPV CSGTCQPTQF RCSNGCCIDS FLECDDTPNC PDASDEAACE KYTSGFDELQ RIHFPSDKGH CVDLPDTGLC KESIPRWYYN PFSEHCARFT YGGCYGNKNN FEEEQQCLES CRGISKKDVF GLRREIPIPS TGSVEMAVAV FLVICIVVVV AILGYCFFKN QRKDFHGHHH HPPPTPASST VSTTEDTEHL VYNHTTRPL |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SPINT1重组蛋白的模拟参考文献示例(内容基于公开研究领域知识，非真实文献)：
---
1. **Title**: *Recombinant SPINT1 inhibits matriptase activity and suppresses tumor growth in colorectal cancer models*
**Authors**: Smith JL, et al.
**Summary**: 研究通过表达重组SPINT1蛋白，发现其能特异性抑制matriptase的蛋白酶活性，阻断下游ERK信号通路，并在结直肠癌小鼠模型中显著抑制肿瘤生长，提示其潜在治疗价值。
2. **Title**: *Structural and functional characterization of the Kunitz domain of SPINT1*
**Authors**: Tanaka K, et al.
**Summary**: 通过X射线晶体学解析SPINT1重组蛋白的Kunitz结构域三维结构，揭示其与靶蛋白酶结合的分子机制，并验证其抑制HGF激活通路的功能，为设计相关抑制剂提供结构基础。
3. **Title**: *SPINT1 recombinant protein ameliorates skin inflammation in Netherton syndrome models*
**Authors**: García A, et al.
**Summary**: 利用重组SPINT1蛋白治疗Netherton综合征模型小鼠，结果显示其通过抑制KLK5蛋白酶活性，减少皮肤屏障损伤和炎症反应，证明其在遗传性皮肤病中的治疗潜力。
---
注：以上文献为示例性内容，实际引用时请以真实发表的论文为准。建议通过PubMed或Web of Science以关键词“SPINT1 recombinant”检索最新研究。
SPINT1 (Serine Peptidase Inhibitor, Kunitz Type 1), also known as HAI-1 (Hepatocyte Growth Factor Activator Inhibitor-1), is a transmembrane protein that functions as a potent inhibitor of several serine proteases. It belongs to the Kunitz-type serine protease inhibitor family and plays a critical role in regulating proteolytic cascades involved in diverse physiological processes, including tissue remodeling, epithelial barrier integrity, and cellular signaling. SPINT1 is synthesized as a single-pass type I membrane protein, containing two extracellular Kunitz domains that mediate its inhibitory activity. Its primary targets include matriptase, hepsin, and hepatocyte growth factor activator (HGFA), enzymes implicated in extracellular matrix degradation, growth factor activation, and cancer progression.
Recombinant SPINT1 protein is engineered to study its biochemical interactions, therapeutic potential, and disease mechanisms. Produced via heterologous expression systems (e.g., mammalian cells or bacteria), it retains the functional domains required for protease inhibition. Researchers utilize this protein to investigate SPINT1's role in regulating protease-dependent pathways, such as the HGF/MET signaling axis critical for wound healing and cancer metastasis. Dysregulation of SPINT1 is linked to congenital disorders (e.g., congenital sodium diarrhea) and cancers (e.g., colorectal, breast), where its loss or mutation disrupts protease balance, promoting invasive growth.
In drug discovery, recombinant SPINT1 aids in screening protease inhibitors or developing biologics targeting aberrant proteolytic activity. Its structural and functional characterization also supports efforts to design synthetic analogs mimicking its inhibitory properties. By modulating protease networks, SPINT1-based research contributes to understanding tissue homeostasis and developing therapies for diseases driven by uncontrolled proteolysis.
×
