关键信息
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基因名
PKNOX1
- 应用
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别名
PKNOX1;PREP1;Homeobox Protein PKNOX1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P55347
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表达区间
1-436aa
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氨基酸序列
MGSSHHHHHH SSGLVPRGSH MMATQTLSID SYQDGQQMQV VTELKTEQDP NCSEPDAEGV SPPPVESQTP MDVDKQAIYR HPLFPLLALL FEKCEQSTQG SEGTTSASFD VDIENFVRKQ EKEGKPFFCE DPETDNLMVK AIQVLRIHLL ELEKVNELCK DFCSRYIACL KTKMNSETLL SGEPGSPYSP VQSQQIQSAI TGTISPQGIV VPASALQQGN VAMATVAGGT VYQPVTVVTP QGQVVTQTLS PGTIRIQNSQ LQLQLNQDLS ILHQDDGSSK NKRGVLPKHA TNVMRSWLFQ HIGHPYPTED EKKQIAAQTN LTLLQVNNWF INARRRILQP MLDSSCSETP KTKKKTAQNR PVQRFWPDSI ASGVAQPPPS ELTMSEGAVV TITTPVNMNV DSLQSLSSDG ATLAVQQVMM AGQSEDESVD STEEDAGALA PAHISGLVLE NSDSLQ
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分子量
48 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
PKNOX1, a member of the homeobox gene family, plays a crucial role in the regulation of gene expression during development and cellular differentiation. Research has shown that PKNOX1 is involved in various biological processes, including neuronal development and cardiovascular formation. Abnormalities in PKNOX1 expression have been linked to several diseases, such as cancer and congenital heart defects, highlighting its importance in maintaining normal physiological functions. The recombinant expression of PKNOX1 protein is essential for understanding its structural and functional properties, which can provide insights into its mechanisms of action and interactions with other cellular components. By producing this protein in a controlled laboratory setting, researchers aim to investigate its role in transcriptional regulation and to explore potential therapeutic applications. The study of PKNOX1 recombinant protein not only contributes to our understanding of developmental biology but also holds promise for the development of targeted therapies for diseases associated with its dysregulation. As such, ongoing research focuses on optimizing expression systems and purification methods to obtain functional PKNOX1 protein for downstream applications in both basic and applied biomedical research.
