关键信息
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基因名
MKRN1
- 应用
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别名
(RING finger protein 61;RING-type E3 ubiquitin transferase makorin-1)
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9UHC7
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表达区间
1-482 aa
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氨基酸序列
MAEAATPGTTATTSGAGAAAATAAAASPTPIPTVTAPSLGAGGGGGGSDGSGGGWTKQVTCRYFMHGVCKEGDNCRYSHDLSDSPYSVVCKYFQRGYCIYGDRCRYEHSKPLKQEEATATELTTKSSLAASSSLSSIVGPLVEMNTGEAESRNSNFATVGAGSEDWVNAIEFVPGQPYCGRTAPSCTEAPLQGSVTKEESEKEQTAVETKKQLCPYAAVGECRYGENCVYLHGDSCDMCGLQVLHPMDAAQRSQHIKSCIEAHEKDMELSFAVQRSKDMVCGICMEVVYEKANPSERRFGILSNCNHTYCLKCIRKWRSAKQFESKIIKSCPECRITSNFVIPSEYWVEEKEEKQKLILKYKEAMSNKACRYFDEGRGSCPFGGNCFYKHAYPDGRREEPQRQKVGTSSRYRAQRRNHFWELIEERENSNPFDNDEEEVVTFELGEMLLMLLAAGGDDELTDSEDEWDLFHDELEDFYDLDL
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分子量
59.4 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
MKRN1 (Makorin Ring Finger Protein 1) is an E3 ubiquitin ligase that plays a critical role in cellular processes by regulating protein stability and turnover through ubiquitination. Research into MKRN1 has garnered attention due to its involvement in various biological functions, including cell proliferation, differentiation, and apoptosis. Additionally, MKRN1 has been implicated in several diseases, particularly in cancer, where its aberrant expression can affect tumor growth and progression. The protein is characterized by its RING domain, known for its enzymatic activity, which enables the transfer of ubiquitin from E2 ubiquitin-conjugating enzymes to substrate proteins. Understanding the structure and function of MKRN1 is essential for elucidating its mechanisms in disease processes. Studies involving MKRN1 recombinant proteins have facilitated the exploration of its interaction with other cellular components and the identification of potential therapeutic targets. In this context, the generation of MKRN1 recombinant proteins is paramount for investigating its role in ubiquitination pathways and for developing novel therapeutic strategies against diseases linked to its dysfunction. Given the protein’s significance, ongoing research aims to clarify its biological functions and therapeutic potential, thereby contributing to a broader understanding of molecular mechanisms in health and disease.
